Effect of Yanghe Huayan Tang Combined with PI3K Inhibitor on Expressions of HER-2, PI3K and p-Akt in PI3K/Akt Pathway of High-expression HER-2 Breast Cancer Cell Lines
10.13422/j.cnki.syfjx.20190425
- VernacularTitle: 阳和化岩汤对HER-2高表达型乳腺癌细胞PI3K/Akt通路HER-2,PI3K,p-Akt表达的机制
- Author:
Nan WANG
1
;
Xiao-fei LIU
1
;
Yang ZHANG
1
;
Qing ZHU
1
Author Information
1. School of Traditional Chinese Medicine(TCM), First Clinical Medical College, Shandong University of TCM, Ji'nan 250014, China
- Publication Type:Research Article
- Keywords:
Yanghe Huayan Tang;
human epidermal growth factor receptor-2(HER-2) high-expression breast cancer;
phosphatidylinositol 3-kinase (PI3K) inhibitor;
vasculogenesis and invasion;
PI3K/serine/threonine kinase (Akt) pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):98-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate effect of Yanghe Huayan Tang and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 in intervening expressions of human epidermal growth factor receptor human epidermal growth factor receptor-2(HER-2), PI3K and phosphorylated serine/threonine kinase (p-Akt)in PI3K/Akt pathway of breast cancer cell line SK-BR-3 (HER-2 high expression) in vitro, and study intervention mechanism of Yanghe Huayan Tang. Method: Yanghe Huayan Tang intestinal absorption fluid was prepared, breast cancer cell strain SK-BR-3 was divided into blank group, Yanghe Huayan Tang group, LY294002 group, LY294002+Yanghe Huayan Tang group. The concentration was respectively 125 g·L-1 for Yanghe Huayan Tang, 0.05 g·L-1 for LY294002, 125 g·L-1 for Yanghe Huayan Tang+LY294002.The expressions of HER-2, PI3K and p-Akt protein were detected by immunohistochemistry and Western blot after 24 h. The most appropriate and effective concentration was obtained through extensive experiments. The expressions of HER-2, PI3K and p-Akt were detected by reverse transcription polymerase chain reaction (RT-PCR). Result: Compared with blank group, LY294002 group, and LY294002+Yanghe Huayan Tang group could inhibit protein and mRNA expressions of HER-2, PI3K, p-Akt in breast cancer cell lines(P<0.05). Yanghe Huayan Tang group could inhibit expressions of HER-2, p-Akt and mRNA (P<0.05) in breast cancer cell lines, but inhibitory effect on PI3K was not obvious; combination with LY294002 could further inhibit PI3K expression compared with single administration with LY294002 (P<0.05). Compared with LY294002 group, LY294002+Yanghe Huayan Tang group had a more obvious inhibitory effect. The combined administration of Yanghe Huayan Tang and LY294002 could inhibit protein and mRNA expressions of HER-2, PI3K, p-Akt significantly (P<0.05). Conclusion: Yanghe Huayan Tang can inhibit angiogenesis and invasion of breast cancer. Its main mechanism is expressed by intervening PI3K/Akt pathway, reducing expressions of HER-2, PI3K and p-Akt in pathway.
