Inhibition Effect of Weichang'an Granule and Decoction on Tumor Growth and Lymph Node Metastasis by RUFY3, SNAI1, VEGF, EMT Related Proteins in Nude Mice with Subcutaneous Xenograft Tumor of Human Gastric Carcinoma MKN45
10.13422/j.cnki.syfjx.20190522
- VernacularTitle: 胃肠安颗粒剂与汤剂抑制人胃癌裸鼠皮下移植瘤生长、淋巴结转移作用比较及对RUFY3,SNAI1,VEGF和EMT蛋白的影响
- Author:
Jia LI
1
;
Wei-xia CHEN
1
;
Zhao-yan LI
1
;
Ai-guang ZHAO
1
Author Information
1. Longhua Hospital, Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 200032, China
- Publication Type:Research Article
- Keywords:
Weichang'an granule;
human gastric cancer cell;
subcutaneous transplanted tumor;
lymph node metastasis;
RUN and FYVE domain-containing protein 3(RUFY3);
Zinc finger protein1(SNAI1);
vascular endothelial growth factor(VEGF);
epithelial-mesenchymal transition(EMT)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):92-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of Weichang'an on the tumor growth and lymph node metastasis, and RUN and FYVE domain-containing protein 3(RUFY3),Zinc finger protein 1(SNAI1),vascular endothelial growth factor(VEGF),epithelial-mesenchymal transition(EMT)-related proteins in nude mice with subcutaneous xenograft tumor human gastric carcinoma MKN45, so as to discuss the mechanism of Weichang'an on MKN45 human gastric metastasis. Method: The nude mice model of human gastric carcinoma MKN45 cells was established and randomly divided into normal saline group (0.5 mL/mouse), Weichang'an granule group (3.54 g·kg-1) and Weichang'an decoction group (35.49 g·kg-1). The tumor weight and volume of axillary lymph nodes in each group were observed. The morphology of lymph nodes in each group was detected by hematoxylin-eosin (HE) staining. The expressions of related proteins were detected by immunohistochemistry, including RUFY3,SNAI1,VEGF,E-cadherin,N-cadherin,Vimentin. Result: Compared with the normal saline group, the tumor weight and volume of axillary lymph node were decreased (P<0.01). HE staining revealed metastatic tumor cells in all lymph nodes. The positive expression rates of RUFY3, SNAI1, VEGF, N-cadherin, Vimentin in the Weichang'an granule group and Weichang'an decoction group were lower than those in normal saline group (P<0.01), while the positive expression of E-cadherin increased significantly (P<0.01). Conclusion: Both Weichang'an granule and Weichang'an decoction can inhibit the tumor growth and metastasis of axillary lymph nodes, obviously down-regulate the expressions of RUFY3,SNAI1,VEGF,N-cadherin,Vimentin and up-regulate the expression of E-cadherin in human gastric MKN45 subcutaneous transplanted tumor in nude mice. This suggested that Weichang'an may inhibit the tumor metastasis through regulation expressions of RUFY3,SNAI1,VEGF and EMT-related proteins.
