Effect of Houpu Sanwu Tang on Small Intestine Interstitial Cells of Cajal and iNOS in Postoperative Ileus Rats
10.13422/j.cnki.syfjx.20190507
- VernacularTitle: 厚朴三物汤对术后肠梗阻大鼠小肠Cajal细胞及iNOS表达的影响
- Author:
Xiu-rong YANG
1
;
Xiao-hong ZHAO
1
;
Hui CHEN
1
;
Su-ning CHEN
1
;
Fei YU
1
Author Information
1. Shengjing Hospital of China Medical University, Shenyang 110004, China
- Publication Type:Research Article
- Keywords:
Houpu Sanwu Tang;
postoperative ileus (POI);
interstitial cells of Cajal (ICC);
inducible nitric oxide synthase(iNOS)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the effect of Houpu Sanwu Tang on the postoperative ileus (POI), and observe its underlying mechanisms of action on interstitial cells of cajal (ICC) and inducible nitric oxide synthase(iNOS) regulation of POI. Method: Totally 87 healthy adult male SD rats were randomly divided into sham operation group, saline control group and Houpu Sanwu Tang group at low, medium and high doses. Houpu Sanwu Tang low, middle and high dose groups received orally Houpu Sanwu Tang(2.25,4.5,9 g·kg-1); Sham operation group (Sham operation) and saline control group (Saline control) received orally normal saline. Surgical procedure was used to induce the postoperative ileus. Changes in intestinal propulsion rate, intestinal mucosal injury and small intestine expression of c-kit and iNOS among these groups were detected. Result: Intestinal propulsion rate was significantly higher in Houpo Sanwu Tang group than that in Saline control group (P<0.01). Rats in Houpo Sanwu Tang group showed reduced intestinal mucosal injury compared with those in Saline control group. C-kit in Houpo Sanwu Tang groups was significantly increased (P<0.01) at 24 h and 48 h, and iNOS in low-dose group and middle-dose group was significantly reduced (P<0.05,P<0.01) at 12 h and 24 h compared with those in the Saline control group. Conclusion: Houpu Sanwu Tang can improve the intestinal propulsion rate and the recovery in POI rats. The mechanisms are related to the inhibition of the generation of iNOS, the alleviation of inflammatory response, and increase of the number of ICC, so as to ensure its normal function, and improve the intestinal dynamic disorder.
