Neuroprotective Effect and Mechanism of Xiaoyaosan on Lipopolysaccharide-induced Nerve Injury in Rat
10.13422/j.cnki.syfjx.20190536
- VernacularTitle: 逍遥散对LPS所致大鼠神经损伤的保护作用机制
- Author:
Bo-yu SHI
1
;
Rong LIU
1
;
Zhi-li RAO
1
;
Xiao-bo LIU
1
;
Jie LUO
1
;
Ya-fei JI
1
;
Qi LIU
1
;
Nan ZENG
1
Author Information
1. Chengdu University of Traditional Chinese Medicine, the Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine;Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
Xiaoyaosan;
lipopolysaccharide (LPS);
nerve injury;
neuro protection;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):50-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect and mechanism of Xiaoyaosan on lipopolysaccharide(LPS)-induced nerve injury. Method: The 56 rats were randomly divided into control group, sham group, model group, amitriptyline group (10 mg·kg-1), fluoxetine group (10 mg·kg-1), Xiaoyaosan group high and low-dose (30,15 g·kg-1).The nerve injury model rat were established by LPS injection into lateral ventride, rats were administrated for 14 days by gavage. The levels of brain-derived neurotrophic factor (BDNF) and β-nerve growth factor (β-NGF) in serum were detected by enzyme linked immunosorbent assay(ELISA), and the expressions of BDNF, nerve growth factor (NGF), tropomyosin receptor kinase B (TrkB), tropomyosin receptor kinase A (TrkA), cAMP response element-binding protein (CREB) mRNA in hippocampus and cortex were detected by Real-time PCR.Protoin expression of BDNF, TrkB, CREB, p-CREB, postsynaptic density protein 95 (PSD95), synaptophysin (SYP) in hippocampus and cortex were detected by Western blot. Result: Compared with control group, LPS decreased the level of BDNF and β-NGF in serum(P<0.01),reduced the expression of BDNF, NGF, TrkB,TrkA, CREB mRNA and the expression of BDNF, TrkB, CREB, Phospho-CREB (p-CREB), PSD95, SYP in hippocampus and cortex(P<0.05,P<0.01). Compared with model group, levels of BDNF and β-NGF in serum in Xiaoyaosan high and low-dose group were increased significantly (P<0.05,P<0.01),and expressions of BDNF, NGF, TrkB,TrkA, CREB, PSD95, SYP in hippocampus and cortex were increased significantly (P<0.05, P<0.01). Conclusion: Xiaoyaosan has a certain antagonistic effect on LPS inducednerve injury, which suggests that the effect is related to activate BDNF/NGF-TrkB/TrkA-CREB pathway and upregulated the expression of synaptic protein.
