Effect of Shenling Baizhu San on Murine Model of IBD Induced by DSS in Mice by Regulating Autophagy
10.13422/j.cnki.syfjx.20190504
- VernacularTitle: 参苓白术散通过调节肠上皮细胞自噬治疗葡聚糖硫酸钠所致小鼠炎症性肠病
- Author:
Yu YOU
1
;
Yu-hui LIU
2
;
Lin LI
2
;
Wang-di LIAO
1
Author Information
1. Gastroenterology Department of the First Affiliated Hospital Nanchang University, Nanchang 330006, China
2. China Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Shenling Baizhu San;
inflammatory bowel disease (IBD);
cell autophagy;
inflammation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):43-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of Shenling Baizhu San (SLBZS) in treating dextra sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) mice and its relationship with autophagy. Method: SPF BALB/c mice were randomly divided into control group,model group,mesalazine group,low,medium and high-dose SLBZS groups,and autophagy inducer rapamycin group.The IBD mice were fed with 5% DSS in their drinking water for 7 days,and the control mice received only water.SLBZS groups were given SLBZS at doses of 3,6,12 g·kg-1·d-1, positive group was given mesalazine sustained release granules at the dose of 2 g·kg-1·d-1, rapamycin group was given rapamycin at the dose of 4 mg·kg-1·d-1, and control mice was given the same volume of normal saline by gavage.The mice weight,stool occult blood in stool,score of disease activity (DAI),pathological examination of intestinal mucosal lesions integral were observed after 7 days. interleukin(IL)-8 and IL-10 in serum were detected by enzyme\|linked immuno sorbent assay(ELISA), vascular tissue samples were prepared for the detection of tumor neorosis factor-(TNF-α) and IL-1β, and transmission electron microscope and Western blot were used to detect the formation of autophagosomes and the level of autophagy. Result: The body mass decrease, the colon length, disease activity scoring, and histological scoring of SLBZS group were better than those of DSS group. Compared with control group, the level of IL-10 decreased, while the level of IL-8 increased obviously (P<0.01), IL-1 and TNF-α expressions significantly up-regulated(P<0.01), the formation of autophagosome decreased, and LC3-Ⅱ/Ⅰ level down-regulated. Compared with IBD group, SLBZS group showed increase in formation of autophagosome and LC3-Ⅱ/Ⅰ and IL-10, decrease in IL-8(P<0.05,P<0.01), and down-regulation in IL-1 and TNF-α expressions(P<0.01). These results were observed in mesalazine group and rapamycin group. Conclusion: Shenling Baizhu San can significantly inhibit the IBD by regulating autophagy and suppressing inflammation.
