Analysis of Transport Mechanism of Flavanomarein in MDCK Monolayer Cell Model
10.13422/j.cnki.syfjx.20190852
- VernacularTitle: 黄诺马苷在MDCK单层细胞模型上的转运机制分析
- Author:
Zheng RAN
1
;
Yan-li GUO
1
;
Li-feng WANG
2
;
Yong-wei ZHANG
1
;
Lin-lin LI
1
;
Xin LUO
1
;
Xin-min MAO
1
Author Information
1. State Key Laboratory of Pathogenesis, Prevention and Treatment of Central Asian High Incidence Diseases, Xinjiang Medical University, Urumqi 830054, China
2. Basic Medical College, Institute of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China
- Publication Type:Research Article
- Keywords:
flavanomarein;
Madin-Darby canine kidney cells;
monolayer cell model;
transmembrane transport;
phlorizin;
phloretin;
efflux ratio
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):155-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the absorption and transport properties of flavanomarein in the Madin-Darby canine kidney(MDCK) monolayer cell model. Method:Methyl thiazolyl tetrazolium(MTT) assay was used to investigate the toxicity of flavanomarein in MDCK cells. The resistance value of MDCK monolayer cell model was detected by Millicell-ERS-2 cell resistometer. The effects of mass concentration of flavanomarein,administration time,sodium-glucose cotransporter(SGLTs) inhibitor and glucose transporter 2(GLUT2) inhibitor on the transmembrane transport of flavanomarein were investigated. The concentration of flavanomarein was determined by UPLC-MS/MS, and the apparent permeability coefficient(Papp) and the efflux ratio(ER) were calculated. Result:When the concentration of flavanomarein was 5.625-120 mg·L-1, there was no significant toxic effect on MDCK cells. The transport of flavanomarein in MDCK monolayer cell model was time-dependent and concentration-dependent. The Papp values of flavanomarein were basically between 1×10-6 cm·s-1 to 10×10-6 cm·s-1. Compared with the blank group, the phlorizin group significantly reduced the transport of flavanomarein on the MDCK monolayer cell model at 60 min and 90 min. Conclusion:Flavanomarein is a moderately absorbed drug in the intestine, its transmembrane transport mechanism is dominated by passive transport along with active transport. SGLTs may be involved in mediating the transport of flavanomarein on the MDCK monolayer cell model.
