Investigation of Integrated Pharmacokinetics of Active Components in Moutan Cortex and Its Correlation with Pharmacodynamics
10.13422/j.cnki.syfjx.20190646
- VernacularTitle: 牡丹皮药效组分的整合药代动力学考察及其与药效的相关性分析
- Author:
Mi-mi LU
1
;
Lu PANG
2
;
Pei CHENG
1
;
Jiang MENG
1
;
Shu-mei WANG
1
Author Information
1. Engineering Technology Research Center for Chinese Materia Medica Quality of Universities in Guangdong Province, Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica, State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. Nanyang Vocational College, Nanyang 474500, China
- Publication Type:Research Article
- Keywords:
Moutan Cortex;
integrated pharmacokinetics;
pharmacodynamics;
correlation analysis;
enzyme-linked immunosorbent assay;
pharmacokinetics-pharmacodynamics;
syndrome of blood-heat and blood stasis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):148-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study on the correlation between integrated pharmacokinetics and pharmacodynamic effects of five active components(oxidized paeoniflorin,paeoniflorin,quercetin,gallic acid, paeonol) in Moutan Cortex. Method:Rats were divided into blank group,model group(syndrome of blood-heat and blood stasis) and drug-administered group.The concentration of five active components in serum were detected with UPLC-MS at different time points after being administrated ethanol extract of Moutan Cortex.The integrated concentrations were calculated according to area under the curve(AUC) self-defined weighting coefficiency.At the same time,the enzyme-linked immunosorbent assay(ELISA) was used to determine the contents of thromboxane B2(TXB2) and 6-keto-prostaglandin F1α(6-keto-PGF1α) in serum at different time points,and then correlation between pharmacodynamics and integrated pharmacokinetics of these five active ingredients was analyzed. Result:At different time points(0.083,0.25,0.5,0.75,1,2,3,4,6,8,10,12 h),the integrated plasma concentrations of these five active ingredients in Moutan Cortex(158.65,174.60,220.13,227.23,244.31,251.51,404.28,654.39,472.62,355.04, 231.56,199.40 mg·L-1) had a good correlation with concentration of TXB2(264.44,261.03,284.93,273.30,264.04, 278.90,274.83,303.58,260.03,264.78,264.40,256.62 μg·L-1) and value of TXB2/6-keto-PGFlα(4.50,4.47,3.66,3.37, 3.29,3.66,3.71,4.30,3.63,3.65,3.75,3.66). Conclusion:There is a good correlation between the dynamic changes in vivo of active components from Moutan Cortex and pharmacodynamic effects of activating blood circulation of this herb.
