Effect of Baihe Gujin Tang on LPS-induced Acute Lung Injury in Mice Based on Nrf2/Keap1/p62 Signaling Pathway
10.13422/j.cnki.syfjx.20191540
- VernacularTitle: 基于Nrf2/Keap1/p62信号通路探讨百合固金汤对LPS诱导的小鼠急性肺损伤的保护作用
- Author:
Min ZHANG
1
;
Cheng-chen XU
1
;
Ting-zhen XU
1
;
Chun-ge DONG
1
Author Information
1. The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310000, China
- Publication Type:Research Article
- Keywords:
acute lung injury;
Baihe Gujin Tang;
nuclear factor E2 related factor 2(Nrf2);
Kelch-likeECH-associated protein 1;
p62;
autophagy
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect and mechanism of Baihe Gujin Tang on lipopolysaccharide induced acute lung injury (LPS-ALI). Method:KM mice were randomly divided into 5 groups:blank control group, model group, dexamethasone 0.002 g·kg-1 group, Baihe Gujin Tang (0.417, 1.25 g·kg-1) group. Except for the blank control group, the other groups were given LPS to induce the mouse ALI model. Except for the blank control group and the model group, the other groups were continuously given intragastric administration for 7 days on the 1st to 7th days before modeling. The lung tissue of the mice was taken 6 h after modeling, and the wet/dry mass ratio (W/D) of the left lung was measured. The serum levels of superoxide dismutase(SOD), malondialdehyde (MDA),reactive oxygen species (ROS)and nitric oxide (NO) were detected in the mice. Thepathological changes of the lung tissues were observed by hematoxylin-eosin(HE) staining. The expression levels of nuclear factor E2 related factor 2(Nrf2), Kelch-likeECH-associated protein 1 (Keap1), p62 and autophagy associated proteinsLC3Ⅱ proteins in the lung tissues were detected by Western blot. Result:Compared with the blank control group, the W/D of the model group was significantly increased (P<0.01), the MDA level in the serum was significantly increased (P<0.01), and the SOD was significantly decreased (P<0.01), lung tissue lesions were severe, and significantly reduced the expression of lung tissue Nrf2, Keap1, but increased p62, LC3Ⅱ protein expression (P<0.01). Compared with model group, the W/D of dexamethasone group and Baihe Gujin Tang 1.25 g·kg-1 group was significantly lower (P<0.01). Baihe Gujin Tang 1.25 g·kg-1 group and dexamethasone group were able to significantly inhibited MDA levels in serum (P<0.05,P<0.01), improved the expression of SOD (P<0.05,P<0.01), dexamethasone group and Baihe Gujin Tang group have different extents of improvement the pathological changes of the lung tissues the pathological changes of the lung tissues, and the lung tissue Nrf2 and Keap1 were significantly increased, and the protein expression of p62, LC3Ⅱwas decreased (P<0.05,P<0.01), each dose group of Baihe Gujin Tang had no effect on LC3B. Conclusion:Baihe Gujin Tang has obvious protective effect on LPS-ALI mice, and its mechanism may be related to the regulation of Nrf2/Keap1/autophagy feedback loop.
