Effect of Modified Xiaoyaosan on ERK Signal Transduction Pathway of Dopamine Receptorin Model Rats with Hyperprolactinemia
10.13422/j.cnki.syfjx.20191640
- VernacularTitle: 逍遥散加减对高泌乳素血症模型大鼠中枢多巴胺受体ERK信号转导通路的影响
- Author:
Lu XU
1
;
Yan LI
2
;
Fang ZHANG
2
;
Jing CHEN
2
Author Information
1. Traditional Chinese Hospital of Liuan, Liuan 237000, China
2. The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, China
- Publication Type:Research Article
- Keywords:
hyperprolactinemia;
Shugan Jianpi method;
extracellularregulated protein kinase(ERK)signaling pathway;
mechanism of action
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):70-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of modified Xiaoyaosan on extracellular regulated protein kinase(ERK) signal transduction pathway of central dopamine receptor in hyperprolactinemia(HPRL)model rats by treating them with Shugan Jianpi as the core. Method:The 96 SD female rats were randomly divided into normal group, model group, Bromocriptine group (0.001 g·kg-1), modified Xiaoyaosan high, medium and low dose group(60,30,15 g·kg-1), dopamine D1 receptor(D1R) antagonist group (SCH23390, SCH23390+ modified Xiaoyaosan group, SCH23390+bromocriptine group), dopamine D2 receptor(D2R) antagonist group (haloperidol group, haloperidol+modified Xiaoyaosan group,haloperidol+bromocriptine group).HPRL rat model was established by pituitary transplanta-tion. After 30 days of administration, renin-angiotensinsystem Ras and Raf protein expressions in thypothalamus of rats were detected by Western blot, and mitogen-activated protein kinase (MAPK) kinase 1/2(MEK1/2)mRNA and extracellular signaling regulates kinase 1/2(ERK1/2) mRNA expressions in the hypothalamus of rats were detected by Real-time PCR.The microstructures of mitochondria in ovarian granulosa cells were observed by electron microscopy. Result:The Ras,Raf protein,MEK1/2 and ERK1/2 mRNA expression were significantly decreased in model group compared with normal group (P<0.01).After SCH23390 antagonistic DIR was applied, Ras, Raf pprotein, MEK1/2 and ERK1/2 mRNA expression in SCH23390 group showed an increased trend compared with model group, and when haloperidol was used to antagonistic D2R, Ras and Raf expression in haloperidol group showed a decreased trend compared with model group, and MEK1/2 and ERK1/2 mRNA expression significantly decreased(P<0.01).The expression of Ras,Raf protein,MEK1/2 and ERK1/2 mRNA was significantly increased after using modified Xiaoyaosan(P<0.01), and the expression was significantly increased in the high and medium-dose groups of prescription(P<0.01). The effect was better than that of bromocriptine group.The morphology of granulosa cells and the number and structure of mitochondria in model group were abnormal under electron microscope. After treatment with modified Xiaoyaosan, the morphology and structure of granulosa cells tended to be normal. Conclusion:Modified Xiaoyaosan stimulates D2R, inhibits D1R, regulates ERK/MAPK signaling pathway.It also effectively improve the mitochondrial function of ovarian granulosa cells and promote follicular development.It is suggested that the modified Xiaoyaosan with a multi-target mechanism in treating HPRL.
