Effect of Modified Ditantang on Autophagy and Relevant Proteins in Brain Cells of Rats with Cerebral Ischemia Reperfusion Injury
10.13422/j.cnki.syfjx.20191504
- VernacularTitle: 加味涤痰汤对脑缺血再灌注损伤大鼠脑细胞自噬相关蛋白表达的影响
- Author:
Wei-da CHEN
1
;
Ting SONG
2
;
Xin ZHAO
2
;
Qiong-yu ZHANG
2
;
Xiao-ting LIU
3
;
Ze-tao CHEN
2
Author Information
1. Shandong University of Traditional Chinese Medicine(TCM), Ji'nan 250355, China
2. Hospital Affiliated to Shandong University of TCM, Ji'nan 250011, China
3. Weifang TCM Hospital, Weifang 261041, China
- Publication Type:Research Article
- Keywords:
modified Ditantang;
cerebral ischemia reperfusion injury;
autophagy;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):64-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of modified Ditantang on autophagy and relevant proteins in brain cells of rats with cerebral ischemia reperfusion injury. Method:The cerebral ischemic reperfusion (CIR) injury model in rats was built by reversible middle cerebral artery occlusion artery suture of middle cerebral embolism method, and randomly divided into sham group, model group, high-dose modified Ditantang group(H-dose), low-dose modified Ditantang group (L-dose, 0.384 g·kg-1) and PLXT group (0.1 g·kg-1). Sham and model groups were given normal saline by gastric perfusion, H-dose and L-dose groups were given modified Ditantang, and the PLXT group were given Piracetam tablets, intragastric volume 10 mL·kg-1. The treatment lasted for 7 d. Within 24 hours after administration, the histopathological examination was performed, the volume of cerebral infarction, neuronal apoptosis and serum levels of inflammatory factors were compared, and the expressions of autophagy related microtuble-associated protein 1 light chain 3(LC3)Ⅱ, Beclin1, B-cell lymphoma-2(Bcl-2) and p62 in brain tissues were determined. Result:Cells and blood vessel necrosis, neuron swelling and interstitial edema were observed in model group, a few neurons died, edema was reduced, swelling of nerve cells was alleviated in H-dose, L-dose and PLXT groups. The volume of cerebral infarction and neuronal apoptosis in H-dose, L-dose and PLXT groups were lower than those in model group (P<0.05). The levels of tumor necrosis factor (TNF)-α, intedeukin (IL)-2 and IL-8 in H-dose, L-dose and PLXT groups were lower than those in model group (P<0.05). Compared with sham group, protein and mRNA expressions of LC3 Ⅱ, Bcl-2 and Beclin1 in the ischemic brain tissue of model group were significantly increased, while protein and mRNA expressions of p62 in the ischemic brain tissue of model group were decreased significantly. Compared with the model group, protein and mRNA expressions of LC3 Ⅱ and Beclin1 in H-dose, L-dose and PLXT groups were significantly decreased, while protein and mRNA expressions of p62 were significantly increased, with significant differences (P<0.05). Conclusion:Modified Ditantang can improve brain injury and interfere with autophagy after MCAO/R, alleviate inflammation, and regulate autophagic activity, which may be related to the down-regulation of expressions of LC3 Ⅱ, Beclin1 and the up-regulation of expression of p62.
