Effect of Shenling Baizhu San on Murine Model of IBD Induced by DSS in Mice Through Regulating of Autophagy
10.13422/j.cnki.syfjx.20191537
- VernacularTitle: 参苓白术散抗炎症性肠病作用与调节肠上皮细胞自噬的关系探讨
- Author:
Yu YOU
1
;
Yu-hui LIU
2
;
Wang-di LIAO
1
Author Information
1. The First Affiliated Hospital of Nanchang University, Nanchang 330006, China
2. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Shenling Baizhu San;
inflammatory bowel disease;
autophagy;
protein pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):51-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Shenling Baizhu San on inflammatory bowel disease (IBD) induced by 5% dextran sodium sulfate (DSS) in mice by regulating autophagy of intestinal epithelial cells. Method:The 84 BALB/c mice were randomly divided into 7 groups with 12 mice in each group. In addition to the normal group, 5% DSS was freely drunk for 7 days to induce acute inflammatory bowel disease. In the treatment group, high, medium and low doses of shenlingbaishu powder (12,6,3 g·kg-1·d-1), mesalazine (2 g·kg-1·d-1) and autophagy inducer rapamycin (4 mg·kg-1·d-1) were given by gavage. Hematoxylin-eosin (HE) staining was used to observe the morphological changes of colon tissues in mice. The autophagosome formation of intestinal epithelial cells was detected by transmission electron microscopy. Western blot was used to detect the ratio of autophagy related protein LC3-Ⅱ/LC3-Ⅰ, phosphatidylinositol-3kinase (PI3K), mammalian target of rapamycin (mTOR), ubiquitin-binding protein-1 (p62), Beclin1 phosphorylation, ULK1, 4EBP protein expression. Result:Compared with normal group, model group mice colonic mucosa epithelial cells are widely missed, most incomplete glands, change in colitis, LC3-Ⅱ content decreased significantly (P<0.05), autophagy is not obvious, and an increased level of autophagy, autophagy pathways in the colon tissue protein PI3K, mTOR, p-p62 phosphorylation degree and the ULK1 protein expression increased significantly (P<0.05)), Beclin1 phosphorylation and 4 ebp protein expression significantly decreased (P<0.05)).Compared with model group, Shenling Baizhu San high dose group, salad lamictal and rapamycin group improved colon tissue obviously, LC3-Ⅱ levels were higher significantly (P<0.05),and Shenling Baizhu San high, medium and low dose group, salad oxazine group and its corresponding inhibitor can obviously inhibit PI3K, mTOR, p-p62 phosphorylation, reduce ULK1 protein expression, promote Beclin1 phosphorylation and 4EBP protein expression (P<0.05). Conclusion:The effect of Shenling Baizhu San on DSS-induced IBD is related to the regulation of the phosphorylation of PI3K, mTOR and p62 proteins in the autophagy pathway of intestinal epithelial cells.
