Effect of Xiao Chaihutang on NF-κB Signaling Pathway in Synovial Tissue of CFA Rats
10.13422/j.cnki.syfjx.20191401
- VernacularTitle: 小柴胡汤对CFA大鼠滑膜组织NF-κB信号通路作用的探讨
- Author:
Li-min WANG
1
;
Jing YU
1
;
Ye-yu ZHAO
1
;
Lin ZHANG
2
;
Li-yan ZHANG
2
;
Wen-na CHEN
2
;
Song GU
2
;
Ming-li GAO
1
Author Information
1. The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine(TCM), Shenyang 110032, China
2. Liaoning University of TCM, Shenyang 110033, China
- Publication Type:Research Article
- Keywords:
Xiao Chaihutang;
complete Freund's adjuvant (CFA) rats;
nuclear transcription factor (NF)-κB signaling pathway;
tumor necrosis factor receptor associated death domain (TARDD);
nuclear transcription factor-κB inhibiting proteinα(IκBα);
IκB kinase-α (IKKα);
NF-κB p65
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):44-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression of tumor necrosis factor receptor-associated death domain (TARDD), nuclear transcription factor-κB inhibiting protein α(IκBα)IκB kinase-α (IKKα) and nuclear transcription factor (NF)-κB p65 protein in the NF-κB signaling pathway of synovial tissues of complete Freund's adjuvant (CFA) rats after treatment with Xiao Chaihutang (XCHT). Method:In animal experiments, SPF health adult female Wistar rats were used to prepare the CFA animal model of rats with rheumatoid arthritis with Freund's complete adjuvant and cattle Ⅱ collagen type. According to the random number table, the rats were randomly divided into the normal group, the model group, the low-dose XCHT group, the medium-dose XCHT group, the high-dose XCHT group, and the Tripterygium glucosides group. The drugs were given at 7 d after the model was built. Both normal group and model group were given water for injection,and low-dose XCHT group(5.94 g·kg-1),medium-dose XCHT group(11.88 g·kg-1),high-dose XCHT group(23.76 g·kg-1),Tripterygium glucosides group(0.006 3 g·kg-1) were given corresponding drugs by gavage for three times a day, 2 mL/time. The histopathology of rat ankle joint was observed, and the protein expressions of TARDD,IKKα,IκBα,NF-κB p65 in the NF-κB signaling pathway in synovial tissue of CFA rats were detected by Western blot. Result:With the increase of the dosage of XCHT, the histopathological score of the right posterior ankle joint of the experimental rats was increased. And in the protein expressions of TARDD,IKKα,IκBα,NF-κB p65 in NF-κB signaling pathway in Synovial Tissue of CFA rats, compared with the model group, the statistical results of the low-dose XCHT group showed decreased protein expressions (P<0.05) and significant differences. However, the statistical results in the medium-dose XCHT group, the high-dose XCHT group and the tripterygium glucosides group showed decreased protein expression (P<0.01) and significant differences. Compared with the normal group, the histopathological score of the right posterior ankle of the model group was significantly increased(P<0.01), and the protein expressions of TARDD, IKKα, IκB α, NF-κB p65 in the NF-κB signaling pathway were significantly increased (P<0.01). Compared with model group, with the increase of dose of Xiao Chaihutang, histopathologic score of posterior ankle of experimental rats significantly reduced (P<0.01). In rats ankle tissue samples, TARDD, IKKα, IκBα, NF-κB p65 key protein expressions in the NF-κB signaling pathway and protein expressions in low-dose XCHT group were obviously lower (P<0.05), and protein expressions in the medium-dose XCHT group, the high-dose XCHT group and the Tripterygium glucosides group were significantly lower (P<0.01). Conclusion:This study shows that as the dose of Xiao Chaihutang increases, it could effectively improve synovitis, and suppress the expressions of key proteins in the inflammatory signaling pathway of NF-κB, thereby preventing inflammation and suppressing bone erosion.
