Advances in Bioculture Technology of Bupleuri Radix and Biosynthesis Pathways of Saikosaponins
10.13422/j.cnki.syfjx.20191048
- VernacularTitle: 柴胡生物培养技术与柴胡皂苷合成生物学的研究进展
- Author:
Chen WANG
1
;
Li-cheng AN
1
;
Jian-chao LI
1
;
Wen-tao QI
1
;
Chang-li LIU
1
;
Li LI
2
Author Information
1. School of Traditional Chinese Medicine(TCM), Capital Medical University, Beijing 100069, China
2. Beijing Institute of Chinese Medicine, Beijing 100035, China
- Publication Type:Research Article
- Keywords:
Bupleuri Radix;
biological culture technique;
saikosaponins;
synthetic biology;
adventitious roots;
culture conditions;
omics
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):228-234
- CountryChina
- Language:Chinese
-
Abstract:
Synthetic biology is an emerging discipline that analyzes the biosynthesis pathways of active constituents in traditional Chinese medicine and explores genes involved in biosynthesis. Bupleuri Radix is one of the most commonly used Chinese medicinal materials with remarkable medicinal value, its index component is saikosaponins, which has significant anti-inflammatory, anti-viral and anti-tumor activities. However, the current wild resources of Bupleuri Radix have been destroyed, and there were some problems in the process of artificial cultivation. The application of biological culture technology and synthetic biology can expand the sources of saikosaponins and protect resources of Bupleuri Radix. The culture conditions of different plants can be followed without a fixed pattern, and the biosynthetic pathways of different medicinal active ingredients are also inconsistent. At present, there is no review report on the culture technology of Bupleuri Radix and the research on the biosynthesis pathway of saikosaponins. This paper introduces the research progress of biological culture techniques, such as callus culture, adventitious root culture, hairy root culture and suspension cell culture used in synthetic biology, and the biosynthesis pathway of saikosaponins and its key enzyme functional genes. It is suggested to optimize the biological culture technology of Bupleuri Radix by referring to the tissue culture technology of other traditional root medicinal materials, so as to provide a reference for the in-depth study on the biosynthesis pathway and metabolic regulation of saikosaponins.
