Secondary Metabolites of Endophytic Fungus Trichoderma harzianum Isolated from Physalis angulata
10.13422/j.cnki.syfjx.20191417
- VernacularTitle: 苦蘵内生真菌Trichoderma harzianum的次级代谢产物
- Author:
Meng-yue TANG
1
;
Jia-jia FAN
2
;
Xia LIU
3
;
Zhi-guo LIU
2
;
Yue-hu PEI
1
;
Yi SUN
2
Author Information
1. Shenyang Pharmaceutical University, Shenyang 110016, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China
- Publication Type:Research Article
- Keywords:
Physalis angulata;
endophytic fungus;
Trichoderma harzianum;
secondary metabolites;
cytotoxicity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):186-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the secondary metabolites of the endophytic fungus Trichoderma harzianum,which was isolated from Physalis angulata. Method:The strains were cultured in a big-scale by oscillating incubator. Then the obtained culture liquid and mycelia were extracted by EtOAC and actone,respectively. The extracts were integrated after recovery of solvents, and then the active secondary metabolites were isolated and purified by comprehensive use of open ODS flash column,sephadex LH-20,HPLC,and LC-MS analysis techniques. Their structures were identified according to their physico-chemical properties and nuclear magnetic resonance (NMR) data. The cytotoxicities of the compounds were determined by methyl thiazolyl tetrazolium(MTT) assay. Result:Ten compounds isolated from T. harzianum were identified as destruxin A2(1),destruxin B2(2),3-isobutyl-pyrrolopiperazine-2,5-dione[cyclo (leu-pro dipeptide)](3),cyclo (Phen-pro) dipeptide(4),cyclonerodiol(5),brevianamide F(6),N-acetyltryptamine(7),9-hydroxyl-(2-methylpropyl) isobutyl phthalate(8),5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone(9),and phenylpropionic acid(10). Compounds 1-10 didn't exhibit significant cytotoxicity to lung cancer cell line A549 in the MTT assay (IC50 ≥ 20 mg·L-1). Conclusion:All compounds were isolated from T. harzianum for the first time.
