Consistency Analysis of “Imprinting Templates” of Chinese Medicine Preparations Based on Dynamic Volatile Components in Houttuyniae Herba
10.13422/j.cnki.syfjx.20190446
- VernacularTitle: 基于鱼腥草挥发性成分动态性的中药制剂“印迹模板”一致性分析
- Author:
Mei-feng XIAO
1
;
Yu-tian ZHANG
1
;
Yan-tao YANG
1
;
Jin ZHOU
1
;
Yi-qun ZHOU
1
;
Hong-yu LIU
2
;
Wen-long LIU
1
;
Fu-yuan HE
1
Author Information
1. Hunan Provincial Key Laboratory of Druggability and Preparation Modification of Traditional Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
2. The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China
- Publication Type:Research Article
- Keywords:
famous classical formulas;
material references;
compound preparations;
homogenization;
total quantum statistical moment;
Houttuyniae Herba;
supramolecular;
“imprinting templates”
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The homogeneity regularity of "imprinting templates" of compound preparation of traditional Chinese medicine(TCM) was revealed, which provided the basis for effectively controlling the quality of compound preparation of TCM, by clarifying the dynamic changeability in number and content of volatile ingredients in different batches of Houttuyniae Herba. Method:The volatile oil of Houttuyniae Herba was extracted by steam distillation.The volatile components in 11 batches of Houttuyniae Herba were determined by GC-MS(electron ionization, ion source temperature of 230℃, detection range of m/z 40-500) combined with literature and ChemicalBook database.The relative content of each component was calculated by peak area normalization method and analyzed by the total quantum statistical moments of fingerprint. Result:There were only 15 common ingredients in the volatile oil from 11 batches of Houttuyniae Herba, accounting for 3.35%of total number and 73.94%of total content, while the number of non-common ingredients was 433, accounting for 96.65%of total number.However, the RSD of total quantum first moment of GC-MS fingerprint was 6.8%, the RSD of total quantum second moment was 14.8%(RSD of standard deviation was 7.4%), and the RSD of similarity of total quantum statistical moment was 3.8%.The type and content of volatile ingredients in Houttuyniae Herba were heterogeneous, but their chromatographic behavior of GC-MS fingerprint was uniform, the whole "imprinting templates" was similar. Conclusion:The total quantum statistical moment(similarity) of TCM fingerprint is applicable to quality control of dynamic Chinese medicines, which will play a breakthrough role in the study of homogeneity and stability of material reference and compound preparation for ancient classical formulas.
