Effect of Intestinal Flora in Rats on Metabolism and Transformation of Effective Components in Polygonum orientale Inflorescences
10.13422/j.cnki.syfjx.20190447
- VernacularTitle: 大鼠肠道菌群对荭草花有效组分代谢转化的影响
- Author:
Shu-ting YANG
1
;
Si-ying CHEN
1
;
Hui-yuan SUN
1
;
Hao CHEN
1
;
Yong HUANG
1
;
Yue-ting LI
1
;
Yong-jun LI
1
;
Lin ZHENG
1
;
Yong-lin WANG
1
Author Information
1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Research Article
- Keywords:
Polygonum orientale inflorescences;
effective components;
mass defect filtration;
intestinal flora;
metabolic pathways;
methylation;
in vitro incubation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):142-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the metabolic characteristics of effective components from Polygonum orientale inflorescences in intestinal flora of rats. Method:The incubating samples of effective components from P. orientale inflorescences in rat intestinal flora in vitro were detected by UPLC-ESI-Q-TOF-MS/MS, the mobile phase was consisted of 0.1%formic acid solution-0.1%formic acid acetonitrile solution and eluted in gradient mode at a flow rate of 0.3 mL·min-1.The mass spectral analysis was detected with electrospray ionization under positive ion mode and negative ion mode.The metabolites and possible biotransformation pathways of effective components form P. orientale inflorescences in rat intestinal flora in vitro was analyzed by Metabolite ToolsTM, mass defect filtration(MDF) and other metabolite analysis techniques and combined with the accurate relative molecular weight of the compounds, the fragment ion information and the literature data. Result:Eighteen metabolites were detected after incubation of effective components from P. orientale inflorescences in rat intestinal flora.The main biotransformation pathways were reduction, oxidation, hydrolysis in Ⅰ phase reaction and methylation in Ⅱ phase reaction. Conclusion:The effective components of P. orientale inflorescences can be transformed into a variety of metabolites under the action of intestinal flora in rats.It is suggested that whether the metabolites are bioactive components should be considered when P. orientale inflorescences is used as medicine.
