Protective Effect of Salvianolic Acid B on Myocardial Injury in Septic Rats
10.13422/j.cnki.syfjx.20191337
- VernacularTitle: 丹酚酸B对脓毒症大鼠心肌损伤的保护作用
- Author:
Xiao-long LIU
1
;
Zi-lin CUI
1
;
Yang LI
1
;
Ya-min ZHANG
1
Author Information
1. Tianjin First Central Hospital, Tianjin 300192, China
- Publication Type:Research Article
- Keywords:
salvianolic acid B;
sepsis;
myocardium injury;
protect effect;
autophagy
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):112-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of salvianolic acid B (Sal-B) on the myocardial apoptosis in rats with sepsis. Method:The 50 male SD rats were randomly divided into sham operation group, model group (cecal ligation and perforation were performed to replicate the animal model of sepsis)and Sal-B low, medium and high-dose group(6, 12, 24 mg·kg-1).The myocardial necrosis markers troponin T(TnT), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB) and the tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were assayed by enzyme-linked immunosorbent assay(ELISA).The antioxidant enzyme superoxide dismutase (SOD) activities and malonaldialdehyde (MDA) levels in myocardial homogenates were determined by spectrophotometrically. Haematoxylin-eosin (HE) staining was used to evaluate the pathological change in rats. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) was used to detect apoptosis of myocardial cells in hearts, respectively. Western blot was used to detect the expression levels of apoptosis-related protein cysteine protease-3 (Caspase-3), b-lymphocytoma-2 (Bcl-2), Bcl-2-related X protein (Bax), autophagy microtubule-related light chain 3 (LC3) and Beclin-1 in myocardial tissue. Result:Compared with sham group, the levels of TnT, CK-MB and cTnI in model group were significantly increased (P<0.01). The levels of TNF-α, IL-1β and IL-6 in myocardial tissue were significantly increased (P<0.01). MDA content in myocardial tissue was significantly increased (P<0.01), SOD activity in myocardial tissue was significantly decreased (P<0.01), the ratio of TUNEL positive myocardial cells was significantly increased (P<0.01), Bax and Caspase-3 protein expression levels were significantly increased, while Bcl-2 expression was significantly decreased (P<0.01), autophagy related protein LC3 and Beclin-1 expression levels were significantly increased (P<0.01). Compared with model group, the levels of TnT, CK-MB and cTnI in Sal-B medium and high-dose groups were significantly decreased (P<0.01), the levels of TNF-α, IL-1 and IL-6 in the myocardial tissue were significantly decreased (P<0.01), MDA content in myocardial tissue was significantly decreased (P<0.05, P<0.01), and the SOD activity in myocardial tissue was significantly increased (P<0.05, P<0.01). The ratio of TUNEL positive cardiomyocytes was significantly decreased (P<0.05), Bax and Caspase-3 protein expressions were decreased, and Bcl-2 expression was significantly increased (P<0.05, P<0.01). The autophagy response was enhanced, the LC3Ⅱ/LC3Ⅰ ratio was increased, and Beclin-1 expression was significantly up-regulated (P<0.05, P<0.01). Conclusion:Sal-B showed a protective effect on the myocardial apoptosis in septic rats maybe by depressing inflammatory infiltration, anti-oxidative effects and improving the exceptional expression of the proteins such as Bax, Bcl-2, Caspases-3 by enhancing the level of autophagy.
