Effect of Ershiwuwei Zhenzhu Tablets on Microvascular Function in Zebrafish
10.13422/j.cnki.syfjx.20190801
- VernacularTitle: 二十五味珍珠片对斑马鱼微血管功能的影响
- Author:
Wei-wu CHEN
1
;
MA Zhuo-ma BAI
2
;
Sheng-ya GUO
3
;
Yi-qiao XU
3
;
Sheng-jun ZHOU
2
;
Lun-ju CHEN
2
Author Information
1. Shenyang Pharmaceutical University, Shenyang 110016, China
2. Tibet Qizheng Tibetan Medicine Co. Ltd, Lhasa 850030, China
3. Hunter Biotechnology Inc., Hangzhou 310051, China
- Publication Type:Research Article
- Keywords:
Ershiwuwei Zhenzhu tablets;
microvascular function;
endothelial injury;
thrombus
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):49-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Ershiwuwei Zhenzhu tablets (EZT) on microvascular function. Method:The zebrafish models of thrombosis, microvascular defect and vascular endothelial injury were induced by using arachidonic acid, simvastatin and ponatinib respectively, and treated with EZT, astragaloside or asprin. To evaluate the protective effect of EZT on vascular endothelium and its effect on thrombus formation, zebrafish heart output and blood flow velocity were counted, and the vascular area of the zebrafish intestine and the intervascular diameter were calculated. The thrombus in the tail vein was observed under microscope. Result:Compared with model group,EZT improved the cardiac output (P<0.05) and blood flow velocity(P<0.01) of zebrafish in thrombus model at a concentration of 27.8 mg·L-1, and promoted angiogenesis in zebrafish at concentrations of 0.11, 0.33, 1 mg·L-1. Compared with the model group, the vascular diameter of the zebrafish internode was significantly increased at the concentrations of 33 mg·L-1 (P<0.05) and 100 mg·L-1 (P<0.01), and the incidence of zebrafish thrombosis was significantly reduced after EZT intervention at 100 mg·L-1(P<0.01). Conclusion:EZT could improve microvascular dysfunction, and its mechanism may be related to the reduction of vascular endothelial damage to promote its angiogenesis and the improvement of microvascular hemodynamics to reduce thrombus formation.
