Effect of Modified Shengjiangsan on Renal Protection for Membranous Nephropathy Rats and Expression of ROS
10.13422/j.cnki.syfjx.20190805
- VernacularTitle: 加味升降散对膜性肾病大鼠肾保护作用及对肾组织线粒体活性氧表达的影响
- Author:
Xin DU
1
;
Guo-dong YUAN
1
;
Ming LIU
1
;
Jin-chuan TAN
1
Author Information
1. Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, China
- Publication Type:Research Article
- Keywords:
modified Shengjiangsan;
membranous nephropathy;
renal protection;
reactive oxygen species(ROS);
foot cell
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):97-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the effect of modified Shengjiangsan on the expression of reactive oxygen species(ROS) in mitochondria of renal foot cells of rats, in order to study the mechanism of modified Shengjiangsan. Method: After be fed for 7 days,the 60 SD male rats were randomly divided into four groups:blank control group, model group, positive medicine group and traditional Chinese medicine(TCM) treatment group. After establishment of the rat model of membranous nephropathy, model group, positive medicine group and TCM treatment group were treated differently. After 4 weeks, all of the rats were put to death, and the expressions of ROS, 24-hour urinary protein quantity,total cholesterol,triglyceride,total protein,albumin,urea nitrogen,creatinine were detected by Real-time fluorescence quantitative polymerase chain reaction Real-time PCR. Result: The expression of 24-hour urinary protein quantity,total cholesterol,triglyceride in positive medicine group and TCM treatment group were reduced,and the expressions of total protein,albumin in positive medicine group and TCM treatment group were reduced compared with those of model group (P<0.05). The expressions of 24-hour urinary protein quantity,total cholesterol,triglyceride,total protein,albumin in TCM treatment group have no difference with those of positive medicine group. After treatment,the expression of ROS mRVA in mitochondria of renal foot cells of rats of positive medicine group and TCM treatment group were increased compared with that of model group (P<0.05),but with no difference in the ROS mRNA expression between positive medicine group and TCM treatment group. Conclusion: Modified Shengjiangsan can effectively control the development of ROS in mitochondria of renal foot cells of rats, and repair the renal function of membranous nephropathy rats by recovering foot cells.
