Effect of Puerarin in Reducing Insulin Resistance in HepG2 Cells via PI3K/Akt/GSK-3β Signal Transduction Pathway
10.13422/j.cnki.syfjx.20191204
- VernacularTitle: 葛根素通过PI3K/Akt/GSK-3β信号通路调节HepG2细胞胰岛素抵抗
- Author:
Zi-yang DAI
1
;
Yu-shan DONG
1
;
Pei-jie DING
1
;
Yu-yang YANG
1
;
Chen-guang DU
1
;
Ya WANG
1
;
Cai-jian HE
1
Author Information
1. North China University of Science and Technology, Tangshan 063210, China
- Publication Type:Research Article
- Keywords:
puerarin;
insulin resistance;
signal transduction;
glycogen synthase kinase-3β (GSK-3β);
hepatic glycogen
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of puerarin on phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) and glycogen synthase kinase-3β (GSK-3β) in insulin resistant HepG2 cells. Method: HepG2 cells were treated with palmitic acid 0.5 mmol·L-1 and insulin 9×10-4 U·L-1 to induce insulin resistant condition for 24 h. Cell viability was detected by methyl thiazolyl tetrazolium (MTT) assay to determine the concentration of puerarin. This experiment included normal control group, model control group and puerarin groups of different doses (40, 80, 160,320 μg·L-1). Glucose detection kit was used to detect the content of glucose in cell culture supernatant. Tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) levels in supernatant of cell culture medium were detected by enzyme-linked immunosorbent assay (ELISA). Hepatic glycogen assay kit was used for detecting the hepatic glycogen content in HepG2 cells. Western blot was applied to detect protein expression levels of PI3K, Akt, p-Akt, GSK-3β and p-GSK-3β. Result: Compared with those in the normal control group, the glucose consumption rate was significantly down-regulated in HepG2 cells in the model control group (P<0.05), the intracellular glycogen content was decreased (P<0.05), the content of TNF-α and IL-6 were increased in supernatant of cell culture medium (P<0.05), PI3K protein level was decreased, Akt phosphorylation level was down-regulated (P<0.05), and GSK-3β protein expression was up-regulated (P<0.05). Compared with those in the model control group, in the puerarin groups, the glucose consumption rate was increased in a dose-dependent manner (P<0.05), the intracellular glycogen content was increased, the contents of TNF-α and IL-6 were reduced in supernatant of cell culture medium (P<0.05), PI3K protein expression was enhanced, Akt phosphorylation level was up-regulated (P<0.05), GSK-3β protein expression was down-regulated, but its phosphorylation inactivation was increased (P<0.05). Conclusion: Puerarin alleviates the insulin resistance of HepG2 cells by strengthening the PI3K/Akt/GSK-3β signal transduction process and increasing the glycogen content in hepatocytes.
