Effect of Chuankezhi Injection on Prevention and Treatment of H1N1 Influenza Virus in Mice
10.13422/j.cnki.syfjx.20191236
- VernacularTitle: 喘可治注射液对甲型H1N1流感病毒FM1株致小鼠肺炎的影响
- Author:
Rong-mei YAO
1
;
Xin MAO
1
;
Tian-ge QU
2
;
Yan-yan BAO
1
;
Jing SUN
1
;
Han SHI
1
;
Yu-jing SHI
1
;
Xiao-lan CUI
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. School of Basic Medicine Science, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Chuankezhi injection;
influenza A H1N1 virus;
viral pneumonia;
pharmacodynamic effect
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):43-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the effect of Chuankezhi injection on mouse model of pneumonia induced by influenza A (H1N1) FM1 strain. Method: ICR mice were randomly divided into normal group, model group, tamiflu control group (27.5 mg·kg-1·d-1) and Chuankezhi injection group (1.5 mL·kg-1·d-1). In the death protection experiment, mice were infected with 2×half lethal dose (LD50) of influenza virus FM1.The Chuankezhi injection was given once a day for 4 days. The number of death animal within 14 days was counted. The mortality and the death protection rate were calculated. In the treatment experiment, mice were infected with 0.8×LD50 of influenza virus, and the Chuankezhi injection was given once a day for 4 days. On the 5th day after the infection, the levels of interleukin-8 (IL-8) in lung, prostaglandin E2 (PGE2) and vasopressin (AVP) in brain were tested by enzyme-linked immunosorbent assay (ELISA). The viral load of influenza virus in lung was tested by Real-time PCR. In the pre-treatment experiment, mice were given Chuankezhi injection once a day for 5 days. 1 hour after the last treatment, mice were infected with 0.8×LD50 influenza virus. 4 days after the infection, the lung index, spleen index, thymus index, and viral load in lung tissue were calculated. Result: Compared with normal group, the IL-8, PGE2 content, lung index and viral load in the lung tissue of model group were significantly increased (P<0.01). Compared with model group, Treatment of Chuankezhi injection could reduce the rate of deaths. Significantly inhibit the level of IL-8, PGE2, and the viral load of influenza(P<0.05,P<0.01). Pre-treatment of Chuankezhi injection could significantly increase the thymus index of infected mice(P<0.01), reduce lung index and the viral load of influenza(P<0.05). Both administration methods can significantly reduce the infiltration of inflammatory cells in the bronchial, perivascular and alveolar interstitium and reduce the exudation of red blood cells in the lumen. Conclusion: Chuankezhi injection could effectively prevent the mouse model of pneumonia induced by influenza A (H1N1) virus. The mechanism might be related to the reduction of inflammation and inhibiting viral replication.
