Effect of Modified Buzhong Yiqitang on Cardiac Function of Diabetic Cardiomyopathy Rats and FABP3 and PPARγ Proteins of Rat Cardiac Myocytes
10.13422/j.cnki.syfjx.20191140
- VernacularTitle: 补中益气汤加减对糖尿病心肌病大鼠心功能及心肌细胞FABP3,PPARγ蛋白表达的影响
- Author:
Jun-tong LIU
1
;
Yu-feng YANG
1
;
Ren-he WANG
1
;
Yan SHI
1
Author Information
1. Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China
- Publication Type:Research Article
- Keywords:
Buzhong Yiqitang;
diabetic cardiomyopathy;
traditional Chinese medicine treatment;
type 2 diabetes mellitus;
fat acid binding protein-3;
peroxisome proliferator-activated receptor gamma
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):35-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of modified Buzhong Yiqitang on cardiac function and fat acid binding protein3 (FABP3)and peroxisome proliferator-activated receptor gamma(PPARγ) protein of cardiac myocytes in type 2 diabetic mellitus (T2DM) rats, and to explore the mechanism of this prescription to protect T2DM cardiomyopathy. Method: After 6 weeks of high-glucose and high-fat diet, streptozotocin (STZ) was intraperitoneally injected (50 mg·kg-1) to establish a diabetic rat model, and then randomly divided into normal group, model group, modified Buzhong Yiqitang group (21 g·kg-1) and metformin hydrochloride group (2 g·kg-1). After continuous administration for 6 weeks, echocardiography was used to detect the cardiac structure and function of the rats in each group. Combined with the measured blood glucose level, it was judged that the rat model building of diabetic cardiomyopathy was successful.Rat heart tissue was dissected, and rat myocardial morphology was observed by hematoxylin-eosin(HE)staining. Western blot was used to detect the expression levels of FABP3 and PPARγ proteins in rat myocardium. Result: Compared with normal group, levels of blood sugar, total triglyceride(TC), triglyceride(TG),insulin (INS) in model group rats increased significantly (P<0.01), slow heart rate, diastolic and systolic left interior diameter (LVIDd)increases, the reduced systolic left ventricular posterior wall thickness, the ratio of peak A to peak E (E/A) ratio, short axial shortening rate(FS)and ejection fraction (EF)were different degree reduced (P<0.01), FABP3 protein expression were significantly elevated, PPARγ protein expression levels were significantly lower (P<0.01). Compared with model group, the situation of blood glucose, TC,TG,INS in modified Buzhong Yiqitang group was significantly improved (P<0.01), cardiomyopathy was significantly improved (P<0.01), FABP3 protein expression was significantly decreased, and PPARγ protein expression was significantly increased (P<0.01). Conclusion: Modified Buzhong Yiqitang can improve glucose and lipid metabolism disorder in diabetic cardiomyopathy rats, improve cardiac function in diabetic cardiomyopathy rats, increase the expression of PPARγ in cardiomyocytes, and reduce the expression of FABP3 protein.
