Protective Effect of Liuwei Dihuangwan on Diabetes Mellitus with Liver Injury Based on SIRT6/NF-κB Signaling Pathway
10.13422/j.cnki.syfjx.20191237
- VernacularTitle: 六味地黄丸基于SIRT6/NF-κB信号通路对糖尿病伴肝损伤的保护作用
- Author:
Hai-ying LU
1
;
Zhi-jie LI
1
;
Shi SHU
2
;
Rui-yi ZHANG
1
;
Peng-yu TAO
1
;
Yue ZHANG
1
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Shanghai Punan Hospital, Shanghai 200125, China
- Publication Type:Research Article
- Keywords:
Liuwei Dihuangwan;
type 2 diabetes mellitus;
liver injury;
silent information regulator 6;
nuclear factor-κB p65
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):28-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective mechanism of Liuwei Dihuangwan on diabetes mellitus with liver injury in terms of inflammation. Method: The 18 db/db mice were selected from animal model of spontaneous type 2 diabetes, mice were randomly divided into model group, Liuwei Dihuangwan group(9.75 g·kg-1·d-1,once a day), resveratrol group(42.6 mg·kg-1·d-1,once a day)based on fasting blood-glucose (FBG). The 12 litter wild db/m mice were randomly divided into normal group, Liuwei Dihuangwan group(9.75 g·kg-1·d-1,once a day). The normal group and the model group were given the same amount of distilled water by gavage. The mice in each group were treated for 16 weeks. FBG, triglyceride (TG) and alanine aminotransferase (ALT) were examined. Histopathological changes were observed by liver biopsy hematoxylin-eosin (HE) staining. Silent information regulator 6 (SIRT6),nuclear factor-kappaB p65 (NF-κB p65),monocyte chemoattractant protein-1 (MCP-1), vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in the liver tissue were detected by Western blot. Result: Compared with normal group, FBG, TG and ALT in model group increased significantly(P<0.01), protein expression levels of NF-κB p65,MCP-1,VCAM-1 and ICAM-1 in model group were significantly up-regulated(P<0.01), the protein expression level of SIRT6 in model group was significantly down-regulated(P<0.01). Compared with model group, Liuwei Dihuangwan and resveratrol could significantly reduce the levels of serum FBG and TG(P<0.01), the protein expression of SIRT6 was significantly increased(P<0.01),NF-κB p65, MCP-1 and VCAM-1 were significantly decreased (P<0.05, P<0.01), and liver steatosis and inflammatory cells infiltration were alleviated. Conclusion: Liuwei Dihuangwan can protect the diabetes mice from liver injury, and its mechanism is related to the improvement of lipid metabolism and the inhibition of inflammatory response.
