Analysis of Effect of Siwutang on Natural Aging Mice Based on Chinmedomics
10.13422/j.cnki.syfjx.20190307
- VernacularTitle: 基于中医方证代谢组学分析四物汤对自然衰老小鼠的干预作用
- Author:
Wang XI
1
;
Qi YAN
1
;
Yan WANG
1
;
Qi-wei LI
1
;
Hua LIANG
1
Author Information
1. Heilongjiang University of Chinese Medicine, Harbin 150040, China
- Publication Type:Research Article
- Keywords:
Siwutang;
aging;
chinmedomics;
blood deficiency syndrome;
glutathione;
natural aging model;
metabolic markers
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):9-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Through metabonomics research methods,the effect of Siwutang on metabolites and metabolic pathways in natural aging mice were observed.The related targets and mechanism of Siwutang intervention in natural aging mice were analyzed. Method: Taking 20-month-old natural aging model mice(equivalent to 60-65 years old of human beings) as the experimental subjects,at the same time,mice aged 3 months were established as the youth group.UPLC-Q-TOF-MS technique was employed to analyze the mouse plasma with mobile phase of acetonitrile(containing 0.1%formic acid)-0.1%formic acid solution for gradient elution and positive ion mode of electrospray ionization,and the metabolic markers were analyzed by principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA),and their metabolic pathways were summarized. Result: Siwutang had obvious reversal effect on the expression levels of 16 aging-related metabolites,among which 9 metabolic markers were statistically significant(P<0.01), mainly affecting glutathione metabolism,pyrimidine metabolism,arginine and proline metabolism,selenium amino acid metabolism and other metabolic pathways. Conclusion: Siwutang can affect the metabolites in the plasma of 20-month-old natural aging mice,and the metabolic disorder during the aging process of mice can be improved by glutathione metabolism,pyrimidine metabolism,selenium amino acid metabolism and other pathways,and this paper can provide biological information for the study of material basis of this compound for aging.
