Comparison of Effect of Fine Powder and Ultrafine Powder of Dendrobium nobile Stem on Intestinal Transporters of Rats
10.13422/j.cnki.syfjx.20190311
- VernacularTitle: 金钗石斛细粉与超微粉对大鼠肠道转运体影响的比较
- Author:
Yi-xin ZHANG
1
;
Hao LIU
1
;
Lei LING
1
;
An-jing LU
1
;
Yao ZENG
1
;
Yu-qi HE
1
;
Yan-liu LU
1
;
Yuan-fu LU
1
Author Information
1. Key Laboratory of Basic Pharmacology and Joint International Research Laboratory of Ethnomedicine, Ministry of Education, Zunyi Medical University, Zunyi 563003, China
- Publication Type:Research Article
- Keywords:
Dendrobium nobile stem;
fine powder;
ultrafine powder;
intestinal transporters;
alkaloids;
multidrug resistance protein;
breast cancer resistance protein
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):86-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the contents of alkaloids from fine and ultrafine powder of Dendrobium nobile stem in rat plasma,and investigate the effect of D. nobile stem with different particle sizes on gene expression of intestinal transporters. Method:Rats were randomly divided into the blank group,fine powder group of D. nobile stem(0.25 g·kg-1) and ultrafine powder group of D. nobile stem(0.25 g·kg-1).The rats were gavaged every 6 h for 5 days.The samples of rat plasma and small intestine were collected.The plasma samples were detected with UPLC-MS.The chromatography separation was performed on a Hypersil Gold C18 column(2.1 mm×150 mm,1.9 μm) with acetonitrile-0.1%formic acid solution as mobile phase for gradient elution.Electrospray ionization (ESI) was applied and operated in positive ion mode.The mRNA expression of multidrug resistance protein 1(MDR1),oligopeptide transporter protein 1(PEPT1),organic cation transporter protein 2(OCT2),breast cancer resistance protein 1(BCRP1),monocarboxylate transport protein 1(MCT1) and multidrug resistance related protein 2(MRP2) in small intestine were quantified by real time fluorescence quantitative polymerase chain reaction. Result:After intragastric administration of fine and ultrafine powder of D. nobile stem,dendrobine,mubironine B and dendramine could be detected in rat plasma.The contents of dendrobine and dendramine in the ultrafine powder group were significantly higher than that in the fine powder group(P<0.05).Compared with the blank group,gene expression of BCRP1 was significantly inhibited by fine and ultrafine powder of D. nobile stem(P<0.05),and the expression in the ultrafine powder group was lower than that in the fine powder group(P<0.05).The gene expression of MDR1 was up-regulated by fine powder of D. nobile stem(P<0.05). Conclusion:Compared with the fine powder group of D. nobile stem,the plasma concentrations of dendrobine and dendramine in the ultrafine powder group are significantly increased,it may be related to the intestinal transporters of MDR1 and BCRP1.These results can provide experimental basis for selecting particle size of D. nobile stem.
