Effect of Zhenxin Xingshui Yizhi Fang and Its Essential Oil Extract on Ability of Learning and Memory in Alzheimer's Disease Model Mice
10.13422/j.cnki.syfjx.20190938
- VernacularTitle: 镇心省睡益智方及其精油对AD模型小鼠学习记忆的影响
- Author:
Jun LUO
1
;
Ke-nan ZHANG
1
;
Shuai XIAO
1
;
Dan-feng TANG
1
;
Lin WU
1
;
Yuan-yuan GUO
1
;
Qin ZHENG
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Zhenxin Xingshui Yizhi Fang;
Alzheimer's disease;
neurotransmitter;
glucose transporter 1;
insulin receptor substrate-1
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):74-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The present study was designed to investigate the modulating effect of Zhenxin Xingshui Yizhi Fang and its essential oil extract on cognitive deficits in mice.Method: For the purpose of this study 5 months old APP/PS1 double transgenic mice and wild-type C57BL/6JNju were selected as experimental animals.Then APP/PS1 double transgenic mice were randomly divided into model group,essential oil low and high-dose groups (12.13,48.50 mg·L-1),Zhenxin Xingshui Yizhi Fang group (0.46 g·kg-1).Meanwhile,wild-type C57BL/6JNju mice were used as a normal group.APP/PS1 double transgenic mice were treated with Zhenxin Xingshui Yizhi Fang and its essential oil extract for 22 consecutive days.Mice were subjected to a Morris water maze test and a platform test in order to determine their cognitive effect.Nissl's staining was used to observe pathological changes in brain tissue.Meanwhile,senile plaques (SP) were observed by employing Thioflavin-S staining.The expression of glucose transporter 1(GLUT1) and insulin receptor substrate-1(IRS-1) were analyzed using immunohistochemistry techniques.The levels of neurotransmitters such as acetylcholine (ACH),glutamate (GLU) and γ-aminobutyric acid (GABA) in the hippocampus were quantified by enzyme-linked immunosorbent assay (ELISA).Result: The memory function was significantly reduced in model group,and severe brain injury and neuronal apoptosis were also observed in comparison to normal group (P<0.05,P<0.01).Compared with the model group,Zhenxin Xingshui Yizhi Fang and its essential oil extract improved previous learning and memory impairments (P<0.05,P<0.01),and the levels of SP,IRS-1,GLU,GABA (except essential oil low dose group) were significantly reduced (P<0.01),meanwhile the levels of ACH and GLUT1 were significantly improved (P<0.05,P<0.01).Conclusion: These results indicate that Zhenxin Xingshui Yizhi Fang and its essential oil extract could ameliorate cognitive deficits and GLUT1 and IRS-1 could be a possible therapeutic target for AD.It may be an interesting approach to the treatment of Alzheimer's disease.
