Effect of Buyang Huanwu Tang on Myocardial Mitochondrial Energy Metabolism and AMPK/PPARα Signaling Pathway in Rats with Diastolic Heart Failure
10.13422/j.cnki.syfjx.20190902
- VernacularTitle: 补阳还五汤对舒张性心衰大鼠心肌线粒体能量代谢及AMPK/PPARα信号通路的影响
- Author:
Zhen WANG
1
;
Jie-bai LI
1
;
Xi DONG
1
;
Xiao-xu SHEN
1
Author Information
1. Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Buyang Huanwu Tang;
rats with diastolic heart failure;
myocardial mitochondria;
energy metabolism;
adenosine monophosphate(AMP)-activated protein kinase(AMPK)/peroxisome proliferators-activated receptors α(PPARα) signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):12-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Buyang Huanwu Tang on myocardial energy metabolism in rats with diastolic heart failure (DHF) based on adenosine monophosphate (AMP)-activated protein kinase (AMPK)/peroxisome proliferators-activated receptors α (PPARα) signaling pathway,and investigate its mechanism of action.Method: The 48 SD rats were randomly divided into sham operation group and model group.DHF rat model was established by abdominal aorta constriction method.The successfully modeled rats were randomly divided into model group,Buyang Huanwu Tang group (12.72 g·kg-1·d-1),metoprolol tartrate group (0.004 5 g·kg-1·d-1),with corresponding drugs in each group by intragastric administration.The sham operation group and model group were given with equal amount of deionized water,once a day.After 8 weeks of continuous drug intervention,the contents of adenosine monophosphate (AMP),adenosine diphosphate (ADP) and adenosine triphophate (ATP) in peripheral blood of rats were determined by enzyme linked immunosorbent assay (ELISA).The changes of myocardial mitochondrial ultrastructure were detected by electron microscope.The protein expression levels of AMPK,PPARα and peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α) in rat myocardium were detected by Western blot.Result: As compared with sham operation group,the contents of AMP and ADP in model group were increased significantly,and ATP content was decreased significantly (P<0.05,P<0.01).As compared with the model group,the AMP content was decreased significantly in Buyang Huanwu Tang group and metoprolol tartrate group (P<0.01),and the content of ADP was decreased while ATP content was increased (P<0.05).As compared with the sham operation group,the number of mitochondria in the model group was decreased,the morphology was abnormal;as compared with the model group,the number of mitochondria in Buyang Huanwu Tang group and metoprolol tartrate group was increased obviously,and the morphology was obviously improved.As compared with the sham operation group,there was no statistically significant difference in the expression of AMPK protein between various groups,but the expression levels of PPARα and PGC-1α protein in the model group were decreased significantly (P<0.05).As compared with the model group,the expression levels of PPARα and PGC-1α protein in Buyang Huanwu Tang group and metoprolol tartrate group were increased significantly (P<0.05).Conclusion: Buyang Huanwu Tang may improve the energy metabolism of the failed heart and delay the progression of heart failure by improving the structure and function of mitochondria,activating AMPK and up-regulating the expression of AMPK/PPARα signaling pathway.
