Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis
10.13422/j.cnki.syfjx.20190936
- VernacularTitle: 芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用
- Author:
Dong-sheng WU
1
;
Hui CAO
2
;
Yu ZHANG
1
;
Ya GAO
2
;
Dong-hua BIN
2
Author Information
1. Hunan University of Traditional Chinese Medicine(TCM), Changsha 410208, China
2. The First Affiliated Hospital of Hunan University of TCM, Changsha 410007, China
- Publication Type:Research Article
- Keywords:
Shaoyaotang;
2,4,6-trinitrobenzenesulfonic acid;
ulcerative colitis;
intestinal mucosal immune barrier;
secretory immunoglobulin A
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):6-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate effect of Shaoyaotang on intestinal mucosal immune barrier induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS) in rats with ulcerative colitis.Method: Sixty SD rats were randomly divided into normal group,model group,mesalazine group (0.067 mg·kg-1),low,medium and high-dose Shaoyaotang groups (1.8,3.6,7.2 g·kg-1).In the TNBS-induced ulcerative colitis model,saline,mesalazine,peony soup were administered by gavage for 7 days.Hematoxylin-eosin (HE) staining was used to detect the histopathological changes of colon tissue.The number of CD4+T lymphocytes and the expression of secretory immunoglobulin A (SIgA) in intestinal mucosa were detected by immunohistochemistry and Western blot.Result: Compared with normal group,the scores of intestinal mucosal injury and the pathological scores in model group increased significantly (P<0.01).The expressions of CD4+T lymphocytes and SIgA in the intestinal mucosa of model group decreased significantly (P<0.01).Compared with model group,the scores of colonic mucosal damage and the pathological scores decreased significantly in rats (P<0.01).The expressions of CD4+T lymphocytes and SIgA in the intestinal mucosa of rats in each group elevated significantly (P<0.05,P<0.01).Compared with mesalazine group and low-dose Shaoyaotang group,the intestinal mucosal injury score and the pathological scores in medium and high-dose Shaoyaotang groups decreased significantly (P<0.01).The expression levels of CD4+T lymphocytes and SIgA protein in the intestinal mucosa of rats in middle and high doses Shaoyaotang groups increased significantly (P<0.05).Conclusion: Shaoyaotang can reduce the intestinal mucosal damage and protect the intestinal mucosal immune barrier by increasing the number of CD4+T cells and the expression of SIgA secretion in the intestinal mucosa.
