Rapid Immunoassay of Aflatoxin B1 and Its Application in Traditional Chinese Medicine
10.13422/j.cnki.syfjx.20190811
- VernacularTitle: 黄曲霉毒素B1免疫快速检测技术研究进展及其在中药中的应用
- Author:
Li-nan SHAN
1
;
Xiao-wen DOU
2
;
Hao LIU
1
;
Ya-ping DUAN
1
;
Shi-hai YANG
1
;
Mei-hua YANG
2
Author Information
1. College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China
2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Under Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
traditional Chinese medicine;
aflatoxin B1;
antibody;
antigen;
immunoassay;
rapid detection
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):194-209
- CountryChina
- Language:Chinese
-
Abstract:
Aflatoxin B1 (AFB1) is a toxic secondary metabolite produced by toxigenic Aspergillus spp. (such as A. flavus and A. parasiticus) with carcinogenic,teratogenic and mutagenic effects. Studies have shown that AFB1 is widely found in crops,food,feed and traditional Chinese medicine,which poses a serious safety hazards to humans healthy. The establishment of a rapid detection technique that is suitable for AFB1 in different matrices has a great significance in preventing contamination,controlling food and medicines safety and ensuring human health. With the continuous improvement of small-molecule immune technology,various rapid immunoassays of AFB1 have been developed and utilized in recent years. This review systematically summarized current relevant standards for the detection of AFB1 in China and the maximum recommended levels for the application of aflatoxins in Chinese herbal medicines in some regions and countries. These standards are mainly applicable for aflatoxins in food,feed and some easily contaminated samples of Chinese herbal medicines. Some studies have shown that except the Chinese herbal medicines specified with the maximum recommended levels,some medicinal herbs and their products were also contaminated by aflatoxins. In addition,this paper reviewed the preparation technology of antigen and antibody for AFB1,and the rapid detection methods based on the specific recognition ability between antigen and antibody, including enzyme-linked immunosorbent assay,immunochromatographic assay,fluorescence immunoassay,chemiluminescence immunoassay,and novel immunosensor method, were also summarized and compared. This review aims to provide the reference for rapid,accurate,and sensitive technical standards for the detection of AFB1 in traditional Chinese medicine during the agricultural planting,distribution, trade and quality supervision and for the market, pharmacies and hospitals,so as to ensure the quality and safety of the traditional Chinese medicines.
