Protective Effect of Gastrodiae Rhizoma Powder with Different Particle Sizes on Cerebral Ischemia Reperfusion Injury in Rats
10.13422/j.cnki.syfjx.20190803
- VernacularTitle: 不同粒径天麻超微粉对脑缺血再灌注脑损伤大鼠的保护作用
- Author:
Ting-ting CHEN
1
;
Fan LIU
1
;
Yan CHEN
1
;
Zi-peng GONG
1
;
Tao TAO
2
;
Ai-min WANG
3
Author Information
1. Guizhou Provincial Key Laboratory of Pharmaceutics, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China
2. People's Hospital of Guizhou Province, Guiyang 550004, China
3. Guizhou Provincial Engineering Research Center for Development and Application of Ethnic Medicine and TCM, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Research Article
- Keywords:
Gastrodiae Rhizoma;
ultramicro-powder;
cerebral ischemia-reperfusion;
cerebral protective effect
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):71-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect of Gastrodiae Rhizoma ultrafine powder with different particle sizes on focal cerebral ischemia-reperfusion injury in rats, in order to provide theoretical basis for the application of ultrafine powder technology in related products. Method: The SD rats were randomly divided into six groups, namely sham-operated group, ischemia-reperfusion group, Gastrodiae Rhizoma ultramicro-powder Ⅰ group(1.2 g·kg-1), Gastrodiae Rhizoma ultramicro-powder Ⅱ group(1.2 g·kg-1), Gastrodiae Rhizoma ultramicro-powder Ⅲ group(1.2 g·kg-1), and nimodipine positive drug group(12.6 g·kg-1). The cerebral ischemia-reperfusion model was established after 7 days of drug administration. The focal ischemia reperfusion injury model was prepared by middle cerebral artery occlusion (MCAO) for 1 h followed by reperfusion for 24 h. The infarct size of brain tissue was measured by triphenyltetrazolium Chloride(TTC) staining. The activities of superoxide dismutase (SOD), malondialdehyde (MDA) and nitricoxide synthase (NOS) in brain homogenate and serum were measured by colorimetry. The contents of glutamate(Glu), aspartic acid(Asp) and glycine(Gly) in hippocampus were determined by liquid chromatography-tandem mass spectrometry (LC/MSMS). Result:The model group showed obvious signs of neurobehavioral deficit (P<0.01) compared with the sham operation group. The area ratio of cerebral infarction was decreased significantly, the contents of Glu, Asp, MDA and the activity of NOS were significantly decreased, while the activity of SOD and content of Gly were significantly increased (P<0.05,P<0.01) in Gastrodiae Rhizoma ultramicro-powder Ⅰ, Ⅱ, Ⅲ groups compared with the model group. SOD activity and Gly content in the Gastrodiae Rhizoma ultramicro-powder Ⅲ group were significantly higher than those in the Gastrodiae Rhizoma ultramicro-powder Ⅰ group and the Gastrodiae Rhizoma ultramicro-powder Ⅱ group (P<0.05), and Glu, Asp, MDA content and NOS activity in the Gastrodiae Rhizoma ultramicro-powder Ⅰ group were significantly lower than those in the Gastrodiae Rhizoma ultramicro-powder Ⅱ group (P<0.05). Conclusion:Gastrodiae Rhizoma ultramicro-powder with different particle sizes has a protective effect on cerebral ischemia-reperfusion injury, and the Gastrodiae Rhizoma ultramicro-powder Ⅲ group has a more obvious protective effect than the the Gastrodiae Rhizoma ultramicro-powder Ⅰ group. The results showed that the protective effect of Gastrodiae Rhizoma ultramicro-powder on middle cerebral artery ischemia-reperfusion in rats was related to its particle size.
