Protective Effect and Mechanism of Extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra on Hippocampal Neurons in Rats with Vascular Dementia
10.13422/j.cnki.syfjx.20190837
- VernacularTitle: 人参-知母-赤芍提取物对血管性痴呆大鼠海马神经元的保护作用及机制
- Author:
Yang-yang GUO
1
;
Zhen-yun HAN
2
;
Dan-feng TIAN
2
;
Dong-hui WANG
1
;
Xue YU
1
;
Jie GUO
1
;
Dan-dan ZHANG
2
;
Yan-shu PAN
1
Author Information
1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2. Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
- Publication Type:Research Article
- Keywords:
extracts from Ginseng Radix et Rhizoma, Anemarrhenae Rhizoma and Paeoniae Radix Rubra;
vascular dementia;
learning and memorizing;
hippocampus;
N-methyl-D-aspartate receptors 1
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):47-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra on N-methyl-D-aspartate receptors(NMDAR1) in hippocampal neurons in rats with vascular dementia and investigate its possible mechanism. Method: The 60 SPF male rats were randomly divided into normal group, sham-operated group,model group, traditional Chinese medicine group(0.20 g·kg-1)and memantine group(2.1 mg·kg-1),with 12 rats in each group. The model was established by repeated ischemia-reperfusion combined with intraperitoneal injection of sodium nitroprusside. After modelling, normal group, sham-operated group and model group were dosed the similar volume of normal saline once a day for 14 days. The learning and memory capacity was assessed by Morris water maze; pathologic change in the CA1 district of hippocampus was assessed by hematoxylin-eosin (HE) staining, and the expression level of NMDAR1 in hippocampal neuron membrane protein was detected by Western blot and immunohistochemistry(IHC),the NMDAR1 mRNA in hippocampal tissue was detected by Real-time PCR. Result:Compared with normal and sham-operated group, the latency period was prolonged in model group(P<0.01), the time in the platform quadrant and the frequency of crossing the platform were lessened significantly(P<0.01), the disorder of the neurons, the decrease of the neuronal number, and the neuronal necrosis and apoptosis were obversed in the CA1 district, expression of membrane NMDAR1 of the hippocampal neuron and NMDAR1 mRNA were increased significantly(P<0.01).Compared with model group, the latency period of extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra group and memantine group were shortened significantly(P<0.05,P<0.01), the time and frequency were increased(P<0.05,P<0.01), the pathologic change was improved markedly, the protein expression of membrane NMDAR1 in hippocampal neuron and NMDAR1 mRNA were decreased significantly(P<0.01). Conclusion:The extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra can improve the learning and memory capacity of rats with vascular dementia, and alleviate the injury in CA1 district of hippocampus. The mechanism may be related to the down-regulation of NMDAR1 expression in hippocampal neurons.
