Effect of Acori Tatarinowii Rhizoma on Intestinal Absorption of Ginsenosides in Dingzhi Xiaowan
10.13422/j.cnki.syfjx.20190552
- VernacularTitle: 使药石菖蒲对定志小丸中人参皂苷类成分肠吸收的影响
- Author:
Ding WANG
1
;
Xiao-chuang LIU
2
;
Juan JI
1
;
Dai-yin PENG
1
;
An ZHOU
1
Author Information
1. Experimental Research Center, Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine, Hefei 230038, China
2. The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China
- Publication Type:Research Article
- Keywords:
Dingzhi Xiaowan;
Acori Tatarinowii Rhizoma;
ginsenosides;
everted gut sac method;
P-glycoprotein;
single pass intestinal perfusion in situ;
verapamil
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):7-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of Acori Tatarinowii Rhizoma on intestinal absorption of ginsenosides in Dingzhi Xiaowan,and reveal the mechanism of Acori Tatarinowii Rhizoma acting as " adjuvant drug" in this formula. Method: The contents of ginsenoside Rg1,Re and Rb1 were measured by UPLC-MS/MS and the absorption of three ginsenosides in different intestine segments was investigated by rat single pass intestinal perfusion in situ,including absorption rate constant(Ka) and apparent permeability coefficient(Papp).Everted intestinal sac model was used to investigate the absorption dosage of three ginsenosides affected by volatile oil from Acori Tatarinowii Rhizoma and verapamil[Ver,a P-glycoprotein(P-gp) inhibitor]. Result:Papp values of three ginsenosides were ≤ 0.191×10-3 cm·min-1 in Dingzhi Xiaowan when lack of Acori Tatarinowii Rhizoma.Compared with lack of Acori Tatarinowii Rhizoma in Dingzhi Xiaowan group,the Ka and Papp values of lack of volatile oil from Acori Tatarinowii Rhizoma in Dingzhi Xiaowan group slightly increased without significant difference in the four intestinal segments,but when the prescription had Acori Tatarinowii Rhizoma,the Ka increased by 3.97-8.35 fold and the Papp increased by 3.99-8.49 fold.The results of everted intestinal sac test showed that volatile oil of Acori Tatarinowii Rhizoma could significantly promote the intestinal absorption of ginsenoside Rg1,Re and Rb1,but there was no dose-dependent. Conclusion:Volatile oil of Acori Tatarinowii Rhizoma can promote the intestinal absorption of three ginsenosides in Dingzhi Xiaowan,and the mechanism may be related to the inhibiting function on P-gp.
