Antibacterial Activity and Chemical Constituents of Sojae Semen Praeparatum
10.13422/j.cnki.syfjx.20190612
- VernacularTitle: 淡豆豉抗菌活性及化学成分分析
- Author:
Bin HU
1
;
Qiu-hong WANG
2
;
Hai JIANG
1
;
Zhi-bin WANG
1
;
Hai-xue KUANG
1
Author Information
1. Key Laboratory of Chinese Materia Medica, Key Laboratory of Basic and Application Research of Northern Medicine under Ministry of Education, Heilongjiang Key Laboratory of Traditional Chinese Medicine(TCM) and Natural Medicine Pharmacodynamic Material Bases, Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
Sojae Semen Praeparatum;
antibacterial activity;
chemical constituents;
isoflavone
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):163-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the antibacterial activity and chemical constituents of Sojae Semen Praeparatum as a traditional Chinese medicine. Method:Sojae Semen Praeparatum was extracted with 80% ethanol,and the extracts were suspended in an appropriate amount of distilled water and extracted with petroleum ether,ethyl acetate and water saturated n-butanol in turn. The antibacterial activity in vitro was used for screening the active fractions. Using activity-guided-isolation,the active fractions were isolated and purified by silica gel column chromatography and preparative HPLC and other methods. Their structures were identified by physicochemical properties and spectroscopic analysis, such as 1H-NMR,13C-NMR and MS. Result:The ethyl acetate fraction of 80% ethanol extract from Sojae Semen Praeparatum showed a stronger activity and was studied further. Eighteen compounds were isolated, and eleven compounds were identified as daidzein (1),daidzin (2),genistein (3),genistin (4),glycitein (5),glycitin (6),daucosterol (7),thymine (8),adenine (9),uracil (10),uridine (11). Conclusion:The ethyl acetate fraction of 80% ethanol extract from Sojae Semen Praeparatum shows an antibacterial activity. Compounds 1-6 are isoflavones. Compounds 7-11 are isolated from Sojae Semen Praeparatum for the first time.
