Serum Pharmacochemical Analysis of Periploca forrestii Rhizomes Based on UPLC-Q-TOF-MS
10.13422/j.cnki.syfjx.20182401
- VernacularTitle: 基于UPLC-Q-TOF-MS技术的黑骨藤血清药物化学分析
- Author:
Xiao-li QIN
1
;
Hao CHEN
1
;
Tao XIA
1
;
Yong HUANG
1
;
Zi-peng GONG
1
;
Yue-ting LI
1
;
Yong-jun LI
1
;
Lin ZHENG
1
Author Information
1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Research Article
- Keywords:
Periploca forrestii rhizomes;
serum pharmacochemistry;
caffeoylquinic acid derivates;
pharmacodynamic material basis;
periplocin;
extract;
metabolic pathways
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):125-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the serum pharmacochemistry of Periploca forrestii rhizomes,and to investigate the pharmacological material basis of extract of P. forrestii rhizomes in rats. Method:Rapid identification of constituents absorbed into blood was carried out by UPLC-Q-TOF-MS,according to retention time,accurate relative molecular mass and standard substance comparison,these constituents were identified and speculated by Data Analysis,Metabolite Detect and other softwares,then preliminary determination of constituents absorbed into blood of rats after oral administration of extract of P. forrestii rhizomes was investigated. Result:Totally 17 constituents absorbed into blood were detected in serum,ten of them were prototype constituents and the other were metabolites.Seven of the prototypes were identified as 5-O-caffeoylquinic acid,4-O-caffeoylquinic acid,3-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid,4,5-di-O-caffeoylquinic acid and periplocin. Conclusion:These constituents absorbed into blood may be substances that act directly in vivo of P. forrestii rhizomes,and it is helpful to clarify pharmacological material basis and mechanism of this herb.
