Effect of Yiqi Jianpi Huayu Recipe Combined with 5-FU on Growth and Immune Function of Subcutaneous Transplanted Tumor in MFC Bearing Mice
10.13422/j.cnki.syfjx.20190521
- VernacularTitle: 益气健脾化瘀方联合5-FU对MFC荷瘤小鼠皮下移植瘤的生长及免疫功能的影响
- Author:
Min WANG
1
;
Shen-lin LIU
1
;
Jian WU
1
;
Hai-dan WANG
1
;
Qing-min SUN
1
;
Bai YE
1
Author Information
1. Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
- Publication Type:Research Article
- Keywords:
gastric cancer;
Yiqi Jianpi Huayu recipe;
5-fluorouracil(5-FU);
MFC cells;
subcutaneous transplanted tumor;
myeloid-derived suppressor cells;
immunocyte
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):65-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the effect of Yiqi Jianpi Huayu recipe (YQJPHY) combined with 5-fluorouracil(5-FU) on the growth and immune function of subcutaneous transplanted tumor in MFC tumor bearing 615 mice. Method:Twenty-four mice were inoculated subcutaneously to establish the transplanted tumor model of gastric cancer in mice, and then randomly divided into model control group, YQJPHY (20 g·kg-1)group, 5-FU (25 mg·kg-1) group and (YQJPHY+5-FU) combined group, with 6 rats in each group. After the last administration, the transplanted tumor, spleen and thymus were stripped completely. The tumor inhibition rate, thymus and spleen index were calculated. Flow cytometry was used to determine the content of myeloid-derived suppressor cells (MDSCs) and its subtype polykaryotype cells (PMN-MDSC), single karyotype cells (M-MDSC) in both peripheral blood and tumor tissue, and macrophages and their M1 type, M2 type, T lymphocyte, B lymphocyte, and natural killer cells (NK cells) in peripheral blood. Expressions of arginase-1(Arg-1) and inducible nitric oxide synthesis (iNOS) gene in tumor tissues were detected by Real-time PCR. Result:Compared with model control group, the weight of mice in YQJPHY group increased, whereas the weight of tumor, the weight of tumor, the index of thymus and spleen decreased in 5-FU group(P<0.05, P<0.01). Compared with 5-FU group, combined group showed gained weight, decreased tumor weight, and increased thymus and spleen index(P<0.05, P<0.01). Compared with model control group, the total MDSCs content in tumor tissue and peripheral blood of the combined group was decreased, and the PMN-MDSC content in 5-FU group and the combined group were decreased(P<0.05, P<0.01). Compared with 5-FU group, the contents of total MDSCs, PMN-MDSC, M-MDSC in combined group were all decreased(P<0.05, P<0.01). Compared with model control group, the number of macrophages and their M1 type in YQJPHY group were higher than those in 5-FU group, while the contents of CD3+,CD4+,CD8+ T cell group decreased(P<0.05, P<0.01). Compared with model control group, gene expressions of iNOS, Arg-1 decreased in all groups(P<0.05, P<0.01). Compared with 5-FU group, gene expressions of iNOS, Arg-1 decreased in combined group(P<0.05, P<0.01). Conclusion:YQJPHY can better inhibit the growth of subcutaneous transplanted tumor when combined with 5-FU, and improve immune status after chemotherapy. The mechanism may be related to the decrease of MDSCs content and the increase of T cell and macrophages content.
