Hepatotoxic Material Basis of Polygoni Multiflori Radix with Zebrafish Model
10.13422/j.cnki.syfjx.20190523
- VernacularTitle: 斑马鱼模型筛选何首乌肝毒性的物质基础
- Author:
Yun-yun QUAN
1
;
Yi-meng ZHOU
1
;
Mei-chen LIU
1
;
Yun-xia LI
1
Author Information
1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources Co-Founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
Polygoni Multiflori Radix;
zebrafish;
hepatotoxicity;
material basis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):52-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the hepatotoxic material basis of Polygoni Multiflori Radix with zebrafish model, in order to provide a theoretical basis for the study on the mechanism of Polygoni Multiflori Radix hepatotoxicity. Method:Emodin, rhein, aloe emodin, emodin-1-O-glucoside, physcion-8-O-glucoside and aloe emodin-8-O-glucoside for three days (at the concentrations of 0.000 73, 0.002 22, 0.015 05, 0.002 36, 0.198 95, 0.072 73 g·L-1) selected from the early stage of the experiment were continuously administered to zebrafish fertilized for 72 hours to establish the liver fluorescence transgenic larvae animal model. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione (GSH) and total bilirubin (TBIL) in zebrafish at 1, 2, 3 day after administration were measured respectively, and the pathological changes of zebrafish liver tissue were analyzed. Result:Emodin, rhein, emodin-1-O-glucoside and physcion-8-O-glucoside had no significant effect on the activities of ALT, AST, GSH and content of TBIL (P<0.01) in zebrafish, and the liver tissue of zebrafish was normal; aloe-emodin could significantly reduce the activities of ALT, AST, GSH (P<0.01), whereas increase the content of TBIL in zebrafish; aloe-emodin-8-O-glucoside could significantly reduce the activities of ALT, GSH, whereas increased the content of TBIL (P<0.05, P<0.01) in zebrafish, with no significant effect on the activity of AST, then both groups showed large flaky necrosis in zebrafish liver tissue, obvious morphologic changes in liver tissue cells, and irregular arrangement of hepatocytes. Conclusion:Aloe-emodin and aloe-emodin-8-O-glucoside in Polygoni Multiflori Radix have a toxic effect on zebrafish liver, suggesting that aloe-emodin and aloe-emodin-8-O-glucoside might be the hepatotoxic material basis of Polygoni Multiflori Radix.
