Mechanism of Modified Tianwang Buxindan on Trx System Oxidative Damage in PCPA Insomnia Model Rats
10.13422/j.cnki.syfjx.20190637
- VernacularTitle: 天王补心丹加减改善PCPA失眠大鼠Trx系统氧化损伤的机制探讨
- Author:
Guang-jing XIE
1
;
Pan-pan HUANG
1
;
Ping WANG
1
Author Information
1. School of Basic Medicine, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China
- Publication Type:Research Article
- Keywords:
insomnia;
Tianwang Buxindan;
supra chiasmatic nucleus (SCN);
oxidative stress;
thioredoxin system
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):32-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of modified Tianwang Buxindan on oxidative damage of Trx system in parachlorophenylalanine (PCPA) insomnia model rats. Method:Sixty male SD rats were randomly divided into blank group and model group. Insomnia model was prepared through intraperitoneal injection with PCPA (150 mg·kg-1). The discontinuous injection lasted for 7 d. After successful modeling, the rats were divided into model group (the same volume of normal saline), low, medium, high-dose Tianwang Buxindan groups (8.8, 17.6, 35.2 g·kg-1·d-1) and estazolam group (0.1mg·kg-1·d-1), with 10 in each group. Autonomous activity video was used to detect circadian activity rhythm. Transmission electron microscopy (TEM) was used to observe supra chiasmatic nucleus (SCN) morphology and Organelle integrity. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expressions of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in serum. Expressions of Trx2, TrxR2 in SCN cells were detected by immunofluorescence (IF) and Western blot. Result:Compared with blank group, the activity rhythm of model group was irregular, the activity time increased (P<0.01), the mitochondrial cristae were broken, the number was reduced, the arrangement was disorder, SOD and GSH-PX decreased, MDA increased (P<0.01), the expression of Trx2 decreased, while the expression of TrxR2 increased (P<0.01). Compared with model group, medium and high-dose Tianwang Buxindan groups could alleviate the circadian rhythm disorder and reduce the activity time (P<0.01), the mitochondrial edema was relieved, part of the cristae were intact, the activities of SOD and GSH-PX were increased, the level of MDA was reduced (P<0.05,P<0.01), expression of Trx2 was significantly increased, and expression of TrxR2 was significantly reduced (P<0.05,P<0.01). Conclusion:The effect of modified Tianwang Buxindan on insomnia model rats is related to the regulation of Trx2 and TrxR2 protein expressions in Trx system.
