Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models Induced by Letrozole
10.13422/j.cnki.syfjx.20191607
- VernacularTitle: 菟丝子总黄酮对来曲唑致多囊卵巢综合征大鼠模型的影响
- Author:
Ming-san MIAO
1
;
Meng-fan PENG
1
;
Xiao-li YAN
1
;
Zheng-wang ZHU
1
;
Ming BAI
1
;
Zhen-zhen WEI
1
;
Meng-yan LI
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450000, China
- Publication Type:Research Article
- Keywords:
polycystic ovary syndrome (PCOS);
dodder total flavone;
hypothalamic-pituitary-ovarian axis;
pathological changes
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(16):17-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by letrozole. Method: Except the blank group, the other rats were given letrozole 1 mg·kg-1 for 21 consecutive days to replicate PCOS animal model. On the 16th day of the modeling, the estrous cycle was detected by vaginal smear, and rat with persistent keratinization of vaginal epithelial cells were selected as the PCOS model rat. The model rats were randomly divided into model group, Dacin-35 group, and high, middle, low-dose dodder total flavonoids groups. The corresponding drugs were given for 21 consecutive days. At the end of the administration, materials were collected to calculate ovary index, and enzyme-linked immunosorbent assay(ELISA) was used to measure estrogen (E2), testosterone (T), gonadotropin-releasing hormone (GnRH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels of serum. The right ovary of rats was stained with haematoxylin-eosin (HE), and the pathological changes were observed by optical microscope. Androgen receptor(AR) expressions in hypothalamus, pituitary and ovary were detected by mmunohistochemistry. Result: Compared with the blank group, serum T, GnRH and LH levels, ovarian index and LH/FSH ratio were significantly increased, while FSH and E2 levels were significantly decreased (P<0.05,P<0.01). Ovarian tissue follicular atresia increased, and the cell layers of follicular membrane were thickened significantly. AR expressions in hypothalamus, pituitary and ovarian tissues were significantly increased in the model group (P<0.01). Compared with the model group, serum E2, T, GnRH and LH levels, ovarian index and LH/FSH ratio were significantly decreased in high, middle and low-dose dodder total flavonoids groups (P<0.01); Serum E2 level was significantly increased (P<0.01), and FSH level was obviously increased (P<0.05) in high and middle-dose dodder total flavonoids group. Serum E2 level in PCOS model rats was obviously increased in low-dose dodder total flavonoids group (P<0.05). The pathological changes of ovarian tissue of cortical thickening and collagenization, atresia follicle increase and corpus luteum decrease of PCOS model rats were alleviated to different degrees in high, middle and low-dose dodder total flavonoids groups. Immunohistochemical results showed that high, middle and low-dose dodder total flavonoids groups could significantly reduce AR expressions in hypothalamus, pituitary and ovary (P<0.01). Conclusion: Dodder total flavonoids may play a protective role in PCOS model rats by regulating the secretion of estrogen and androgen and affecting the hypothalamic-pituitary-ovary axis pathway.
