Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of “Insight into Abdomen”
10.13422/j.cnki.syfjx.20191605
- VernacularTitle: 基于“入腹知性”理论的黄芩干预大鼠肠道菌群多样性的探讨
- Author:
Hong-yu LIU
1
;
Ya-nan ZHANG
1
;
Zi-hui LI
1
;
Ping-ping CHEN
1
;
Hai-xue KUANG
1
;
Shu-min LIU
1
Author Information
1. Heilongjiang University of Chinese Medicine, Key Laboratory of Chinese Materia Medica under Ministry of Education, Heilongjiang Key Laboratory of Traditional Chinese Medicine Pharmacodynamic Material Bases, Center for Drug Safety Evaluation, Research Institute of Chinese Medicine, Harbin 150040, China
- Publication Type:Research Article
- Keywords:
Scutellariae Radix;
intestinal flora;
high-throughput sequencing;
property theory
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):167-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of Scutellariae Radix on the diversity of intestinal flora in rats under physiological conditions, in order to determine the property of Scutellariae Radix property. Method: 16S rRNA high-throughput gene sequencing technique was used to detect the cecum solutes of rats treated with Scutellariae Radix (10 g·kg-1). The number, richness and diversity index of the intestinal flora taxon (OTUs) and the differential phylum and genus were comprehensively analyzed. The network visualization was used to find the correlation between differential phylum and genus. Result: Based on the Illumina Miseq platform, compared with the blank group, the number of OTUs and the index of richness and diversity of the intestinal flora of the rats treated with Scutellariae Radix decreased. Bacteroidetes and Spirochaetes were significantly up-regulated(P<0.01), while Firmicutes, Actinobacteria, Proteobacteria and Tenericutes were significantly down-regulated(P<0.01). Among the 24 differential bacteria genera, 17 were significantly down-regulated(P<0.05, P<0.01), while 7 species were significantly up-regulated(P<0.01). On the basis of the network visualization analysis, the most influential phylua were Bacteroides and Firmicutes, in which Firmicutes showed a negative correlation with the rest of phylum, with the strongest negative correlation with spirochetes. There was a positive correlation between Bacteroides, Proteobacteria and Firmicutes, but a negative correlation with Actinobacteria, and no correlation with other phylum. Most of the differential bacteria were enriched in Ruminococcus, Paraprevotella, Prevotella and Oscillospira. Conclusion: Scutellariae Radix can reduce the diversity of intestinal flora and inhibit the metabolism of the body, so its property is cold.
