Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS
10.13422/j.cnki.syfjx.20191815
- VernacularTitle: 国内外产西洋参挥发性成分的HS-SPME/GC-MS比较
- Author:
Yu SI
1
;
Yun-he LIU
1
;
Zhong-yao WANG
1
;
Jin-ping LIU
1
;
Ping-ya LI
1
;
Yu-feng JIAO
1
Author Information
1. Natural Medicine Research Center, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China
- Publication Type:Research Article
- Keywords:
Panacix Quinquefolii Radix;
volatile components;
gas chromatography-mass spectrometry;
headspace solid phase microextraction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):130-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the volatile chemical constituents of Panacix Quinquefolii Radix of different origins and different growth years,in order to provide the theoretical basis for the further development and utilization of Panacix Quinquefolii Radix. Method: Headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to extract,analyze and identify the volatile constituents of Panacix Quinquefolii Radix. The chromatographic peak area normalization method was used to determine the relative amount of ingredients. Result: A total of 151 compounds were identified in Panacix Quinquefolii Radix samples,including 99 hydrocarbons,21 alcohol phenols,7 aldehydes,8 ketones,1 ester,15 heterocyclics and other compounds. Totally 68 kinds of compounds were identified in the roots of three-year-old Jilin Baishan,and the mass fraction accounted for 98.27%,67 compounds were identified in the roots of four-year-old Jilin Baishan,and the mass fraction accounted for 98.79%,65 compounds were identified in 3-year-old Panacix Quinquefolii Radix root,and the mass fraction accounted for 95.81%,and 63 compounds were identified in 4-year-old Panacix Quinquefolii Radix root,and the mass fraction accounted for 99.67%. The specific components of the volatile oil components of the four Panacix Quinquefolii Radix samples were 24,23,19,and 23,respectively,and the total composition was 16 species. Conclusion: The content and composition of volatile chemical constituents of Panacix Quinquefolii Radix of different origins are very different. This experiment provides a reference for the future quality evaluation of Panacix Quinquefolii Radix medicinal materials,rational development and utilization of resources.
