Intervention Effect of Dachuanxiong Fang Multi-component Preparation on Acute Migraine Rat Model by Urine Metabolomics
10.13422/j.cnki.syfjx.20191705
- VernacularTitle: 基于尿液代谢组学技术分析大川芎方多组分中药制剂干预急性偏头痛大鼠模型的影响
- Author:
Shi-yu MA
1
;
Lan SHEN
1
;
Xiao LIN
1
;
Yi FENG
2
Author Information
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 201203, China
2. Engineering Research Center of Modern Preparation Technology of TCM under Ministry of Education, Shanghai University of TCM, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
Dachuanxiong Fang;
multi-component Chinese medicine preparation;
metabolomics study;
acute migraine;
tryptophan metabolism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):101-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the intervention effect of Dachuanxiong Fang multi-component preparation on acute migraine rats, in order to explore the possible mechanism by using urine metabolomics technology. Method: The forty SD rats were randomly divided into 5 groups (n=8), control group, model group, low-dose Dachuanxiong Fang multi-component preparation (DCXF) group (0.19 g·kg-1), medium-dose DCXF group (0.37 g·kg-1), and high-dose DCXF group (0.74 g·kg-1). Rats were subcutaneously injected with 10 mg·kg-1 nitroglycerin for modeling, and Dachuanxiong Fang multi-component preparation was administered through intragastric administration. After half an hour of administration, blood was collected from the abdominal aorta, and the content of 5-hydroxytryptamine (5-HT), 5-hydroxyindole-3-acetic acid (5-HIAA), nitric oxide (NO), nitric oxide synthase (NOS), calcitonlin gene related peplide (CGRP), dopamine(DA) in plasma were determined by enzyme-linked immuno sorbent assay (ELISA) kits. The contents of 5-HT and NOS in the hypothalamus and brainstem of the control group and the model group were analyzed, and the average optical density value was used for statistical analysis. UPLC-TOF/MS combined with principal component analysis (PCA) analysis was used to analyze different groups of rats and discover differential metabolites. Differential metabolites were analyzed by MetaboAnalyst 3.0 software for possible metabolic pathways. Result: After modeling, compared with the control group, the content of neurotransmitters in the model group was significantly increased (P<0.01). After the intervention with Dachuanxiong Fang multi-component preparation, compared with the model group, the content of neurotransmitters was significantly decreased (P<0.05, P<0.01). Based on UPLC-TOF/MS analysis, 10 potential biomarkers were found. After the intervention with Dachuanxiong multi-component preparation, the contents of tryptophan and proline were down-regulated, while the contents of gamma-aminobutyric acid(GABA), lactic acid and β-hydroxybutyrate showed an upward trend. According to the metabolic pathway prediction study, metabolic pathways with a higher correlation were found to be alanine, aspartic acid and glutamate metabolism, propionic acid metabolism, nitrogen metabolism and tryptophan metabolism. Conclusion: The possible metabolism pathway of Dachuanxiong Fang multi-component preparation is mainly amino acid metabolism in urine, and kynurenine is also the product of tryptophan metabolism pathway. The kynurenine metabolic pathway is also one of the main pathways of tryptophan metabolism.
