Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)
10.13422/j.cnki.syfjx.20191703
- VernacularTitle: 拟黑多刺蚁活性组分对系统性红斑狼疮的治疗作用及机制
- Author:
Zhang-zhi MAO
1
;
Jun-hui HE
2
;
Dong-mei LI
2
;
Fei HE
2
;
Guo-shou LU
2
;
Xiao-xi HU
2
;
Gui-ning WEI
2
Author Information
1. Nanning Third People's Hospital, Nanning 530003, China
2. Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning 530022, China
- Publication Type:Research Article
- Keywords:
active fraction of Polyrhachis vicina;
systemic lupus erythematosus (SLE);
Arthus reaction;
CD4+T cells
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):65-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of an active fraction of Polyrhachis vicina (AFPV) on systemic lupus erythematosus (SLE) and its possible mechanism based on animal and cell models. Method: Totally 60 SD rats were randomly divided into normal control group, model group, prednisone acetate group (5 mg·kg-1), and high, medium and low-dose AFPV groups (400, 200, 100 mg·kg-1). SLE model was replicated with bovine serum albumin-Freund's complete (incomplete) adjuvant. Arthus reaction was observed to study the effect of AFPV on the diameter of back skin redness in rats with SLE. The expressions of anti-double-stranded DNA (dsDNA) antibody, complements 3 (C3), complement 4 (C4), immunoglobulin M (IgM), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-31 (IL-31) and interleukin-33 (IL-33) in serum were detected by enzyme-linked immunosorbent assay. CD4+T cells were isolated from the spleens of MRL/lpr and C57BL/6J mice at the age of 16 to 18 weeks by immunomagnetic beads method. The expressions of miR-200a and miR-155 and the levels of zinc-finger-enhancer binding protein 1(ZEB1) and suppressor of cytokine signaling1(SOCS1) in CD4+T cells were observed to explore the effect of AFPV on SLE and its possible mechanism. Result: Compared with the normal group, the diameter of back skin swelling in the model group was significantly increased (P<0.01). Compared with the model group, the high and medium-dose AFPV groups significantly reduce the skin redness on the back of SLE rats (P<0.05, P<0.01); Compared with the normal group, the serum levels of IgM, IL-6 and IL-33 were significantly increased in the model group (P<0.05). Compared with the model group, the serum levels of IgM, IL-6 and IL-33 were significantly decreased after the intervention of AFPV (P<0.05). Compared with the normal group, the expression of miR-200a was significantly decreased (P<0.01), and its target protein ZEB1 was significantly increased (P<0.01) in the CD4+T cells of MRL/lpr lupus mice. Compared with the model group, the expression of microRNA-200a increased significantly, the expression of microRNA-155 decreased significantly (P<0.01), the level of ZEB1 decreased significantly, and the expression of SOCS1 increased significantly after AFPV intervention(P<0.01). Conclusion: AFPV has therapeutic effect on rats with SLE, its mechanism may be related to the regulation of miR-200a/ZEB1 and miR-155/SOCS1.
