Improving Quality Standard of Processed Slices of Paridis Rhizoma of Chinese Pharmacopoeia
10.13422/j.cnki.syfjx.20191617
- VernacularTitle: 关于完善2020年版《中国药典》重楼饮片质量标准
- Author:
Bo-ya JU
1
;
Yan-min LI
2
;
Hou-da ZHU
2
;
Yun-ge FANG
2
;
Rui WANG
1
;
Liang-mian CHEN
2
;
Hui-min GAO
2
;
Zhi-min WANG
2
Author Information
1. Shanxi University of Traditional Chinese Medicine, Taiyuan 030619, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Paridis Rhizoma;
property;
polyphyllin;
quality standard
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):93-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the appearance description,TLC examination and content determination was carried out, in order to improve the quality standard of processed slices of Paridis Rhizoma in the 2020 edition of Chinese Pharmacopoeia. Method:Based on the literature review and observation on the samples,the appearance description was described. TLC examination was used for the qualitative analysis. HPLC was used for the determination of polyphyllin Ⅰ,Ⅱ,Ⅵ and Ⅶ in the commercial and processed samples. UPLC was employed for the determination of 10 steroidal saponins,namely pseudoprotodioscin,polyphyllin Ⅶ,17-hydroxygracillin,polyphyllin H,polyphyllin Ⅵ,polyphyllin Ⅱ,dioscin,gracilin,polyphyllin Ⅰ and polyphyllin Ⅴ. Result:For the appearance description,color and luster,texture,odor and taste as well as the diameter of 1.0-4.5 cm were recorded. polyphyllin Ⅵ was not detected in the thin layer chromatograms of most of the tested samples derived from high-quality species but obviously detected in those of Trillium Rhizoma. Five of 13 commercial samples met the requirements that the total amounts of polyphyllin Ⅶ,Ⅵ,Ⅱ,and Ⅰ should be no less than 0.6%according to the current Chinese Pharmacopoeia. Because softening and drying had the obvious influence on the contents of steroidal saponins in the samples,soaking and sun-drying were preferred. Conclusion:Appearance description should be supplemented. Polyphyllin Ⅵ is not considered as one of quality markers for the TLC identification and HPLC determination of Paridis Rhizoma. Polyphyllin H was considered as a new marker for the quality control. It is recommended that the total amounts of polyphyllin Ⅶ,H,Ⅱ,and Ⅰ should be no less than 1.0%.
