Analysis on “Point-line-surface” Quality Standard of Yangjing Zhongyu Tang
10.13422/j.cnki.syfjx.20191651
- VernacularTitle: 养精种玉汤的“点-线-面”质量标准分析
- Author:
Jun-ping ZHU
1
;
Jia-ming WEI
1
;
Rui-lian LIU
2
;
Hui-hui LIANG
1
;
Ge YU
1
;
Wen WEN
1
;
Yan MAO
1
;
Yi-qun ZHOU
1
;
Wen-long LIU
1
;
Fu-yuan HE
1
;
Xi-li ZHANG
1
;
Long LI
1
Author Information
1. College of Pharmacy, Hunan Provincial Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine(TCM), Laboratory of Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha 410208, China
2. Hunan Academy of Chinese Medicine, Changsha 410006, China
- Publication Type:Research Article
- Keywords:
famous classical formulas;
Yangjing Zhongyu Tang;
quantitative assay of multi-components by single-marker;
correction factor;
total component analysis;
quality standards;
fingerprint
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):12-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Based on the analysis of the total components of fingerprint and the determination methods of the existing components in the 2015 edition of Chinese Pharmacopoeia, the "point-line-surface" quality standard of Yangjing Zhongyu Tang was established by the "point" of each single component (morroniside, loganin, paeoniflorin, ferulic acid and verbascoside) to the "line" of multicomponent and the "face" of fingerprint of the whole component. Method: XB-C18 column (4.6 mm×250 mm, 5 μm) was used for gradient elution of 0.1% phosphoric acid aqueous solution-acetonitrile. The column temperature was 30℃, the injection volume was 10 μL, the flow rate was 1.0 mL·min-1, and the detection wavelengths were 240, 316, 230, 334 nm. The contents of these five components in Yangjing Zhongyu Tang were determined by three correction methods, external standard method and regression equation method. At the same time, the fingerprint of Yangjing Zhongyu Tang were analyzed by total component analysis and similarity evaluation. Result: With ferulic acid as reference, the relative correction factor (f) of morroniside, loganin, paeoniflorin and verbascoside were 0.392 1, 0.421 4, 0.261 7, 0.268 6 by multi-point correction method, and their f (slope correction method) were 0.385 4, 0.419 4, 0.255 9, 0.274 0, respectively. Twenty characteristic peaks of fingerprint were analyzed and the similarity was ≥ 0.999.There was no significant difference in the contents of these five components from Yangjing Zhongyu Tang determined by the quantitative assay of multi-components by single-marker (QAMS) correction method, the external standard method and the regression equation method. Conclusion: The comprehensive quality standard established by the total component analysis of fingerprint combined with various determination methods of existing components in the 2015 edition of Chinese Pharmacopoeia has been validated in famous classical formula of Yangjing Zhongyu Tang, which can provide ideas and methods for the quality control with quantitative determination and fingerprint of other famous classical formulas.
