Chemical Constituents of Ethyl Acetate Extract from Seed Cake of Camellia oleifera and Its Anti-inflammatory Activities
10.13422/j.cnki.syfjx.20191012
- VernacularTitle: 油茶枯乙酸乙酯部位化学成分及其抗炎活性
- Author:
Bing JIAO
1
;
Cheng-ting XU
1
;
Qing LI
1
;
Jiang-ke QIN
1
;
Yun-fei SONG
2
Author Information
1. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China
2. Guilin Layn Natural Ingredients Corporation, Guilin 541199, China
- Publication Type:Research Article
- Keywords:
Camellia oleifera;
seed cake of Camellia oleifera;
ethyl acetate extract;
2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):132-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To intensively study the chemical constituents from the seed cake of Camellia oleifera and its pharmacological activities,in order to provide scientific basic for its further development and utilization. Method: All kinds of column chromatography and spectral methods were employed to isolate and identify the monomeric compounds from its ethyl acetate portion of ethanol extract. The in vitro anti-inflammatory effects were evaluated by LPS-induced inflammatory model in RAW264.7 macrophages. Result: Eight phenolic acids and two flavonoids were isolated from the ethyl acetate soluble portion and identified as p-hydroxybenzoic acid(1),protocatechuic acid(2),gallic acid(3),methyl gallate(4),ethyl gallate(5),isovanillic acid(6),ethyl 3,4-dihydroxylbenzoate(7),2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde(8),quercetin(9),rutoside(10). Among them, compounds 4-8 were first isolated from this plant. These compounds had good anti-inflammatory activities against NO production in LPS-stimulated RAW264.7 macrophages in an obvious dose-dependent manner. Among them, compound 8 showed a strongest activity. Conclusion: The above results show that the phenolic acids and flavonoids from seed cake of C. oleifera have good prospects for the development and application of anti-inflammatory drugs.
