Effect of Coptidis Rhizoma on Gut Microbiota of Normal and Type 2 Diabetic Rats Before and After Being Steamed with Rice Wine Based on 16S rRNA Technique
10.13422/j.cnki.syfjx.20192447
- VernacularTitle: 基于16S rRNA技术分析黄连酒蒸前后对正常及2型糖尿病大鼠肠道微生物的影响
- Author:
Jin WANG
1
;
Qian RAN
1
;
Lin WANG
1
;
Chang-jiang HU
1
;
Qin-wan HUANG
1
;
Hai ZHANG
2
Author Information
1. Key Laboratory of Technology of Chinese Medicine Processing, National Administration of Traditional Chinese Medicine(TCM), School of Pharmacy, Chengdu University of TCM, Chengdu 611137, China
2. Ya'an Xunkang Pharmaceutical Co. Ltd., Ya'an 625600, China
- Publication Type:Research Article
- Keywords:
Coptidis Rhizoma;
raw products;
steamed products;
16S rRNA technique;
type 2 diabetes mellitus;
intestinal microflora;
antioxidant activity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):92-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Coptidis Rhizoma on intestinal microflora of normal and type 2 diabetic rats before and after being steamed with rice wine based on 16S rRNA sequencing technology. Method: The type 2 diabetic rat model was established by high-fat diet and streptozotocin. Normal and model rats were given the decoction of Coptidis Rhizoma or Coptidis Rhizoma steamed with rice wine every day (0.8 g·kg-1, according to raw drug quantity), the positive drug group was given metformin solution every day (0.25 g·kg-1), continuous administration for 30 d. Blood glucose was measured once a week and oral glucose tolerance was measured on the 27th day of administration. On the 30th day, feces were taken and the rats were anesthetized. Blood was taken for enzyme linked immunosorbent assay (ELISA). Colon was dissected for hematoxylin-eosin (HE) staining. Based on 16S rRNA gene sequencing technology, the alpha diversity, beta diversity, abundance and composition, and the correlation with anti-inflammatory and antioxidant activities of intestinal microflora were comprehensively analyzed and evaluated. Result: After giving normal rats Coptidis Rhizoma or Coptidis Rhizoma steamed with rice wine, there were some inflammatory reactions, imbalance of oxidation-antioxidant system, pathological damage of colon and changes of intestinal microflora, which showed some toxic and side effects, but the toxic and side effects of Coptidis Rhizoma steamed with rice wine were lower than those of Coptidis Rhizoma. Coptidis Rhizoma could reduce fasting blood glucose and improve glucose tolerance in type 2 diabetic rats before and after steaming. Although the effect of Coptidis Rhizoma steamed with rice wine had an increasing trend, there was no significant difference between before and after processing. Type 2 diabetic rats appeared mild inflammation and imbalance of oxidation-antioxidant system, characterized by significant increase of contents of interleukin (IL)-6, nuclear transcription factor (NF)-κB and malondialdehyde (MDA) (P<0.05, P<0.01), significant decrease of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) activities (P<0.01). Coptidis Rhizoma could inhibit inflammation and imbalance of oxidation-antioxidant system, and alleviate pathological damage of colon before and after steaming in type 2 diabetic tats, the effect of Coptidis Rhizoma steamed with rice wine was better than that of Coptidis Rhizoma. Significant intestinal flora disorders were observed in type 2 diabetic rats, including down-regulation of Bacteroidetes, Firmicutes and Kiritimatiellaeota, up-regulation of Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, Cyanobacteria and Verrucomicrobia. Coptidis Rhizoma could alleviate intestinal microflora disturbance before and after steaming, and the regulation effect of Coptidis Rhizoma on Kiritimatiellaeota, Cyanobacteria and Verrucomicrobia was stronger than that of Coptidis Rhizoma steamed with rice wine. Coptidis Rhizoma steamed with rice wine could significantly reduce the level of Chloroflexi, but Coptidis Rhizoma had no such effect. The intestinal microflora may be correlated with the antioxidant capacity of Coptidis Rhizoma before and after steaming. Conclusion: Coptidis Rhizoma can treat type 2 diabetic rats by regulating intestinal microflora before and after steaming with rice wine, and the effect of Coptidis Rhizoma steamed with rice wine is better. The toxic and side effects of Coptidis Rhizoma on intestinal flora are reduced by steamed with rice wine. The processing mechanism of increasing efficacy and decreasing toxicity of Coptidis Rhizoma steamed with rice wine is closely related to intestinal microflora.
