Effect of Tangnaikang on Imbalance Between NE and α1-AT in Spontaneous Type 2 Diabetic Mouse
10.13422/j.cnki.syfjx.20191221
- VernacularTitle: 糖耐康对自发性2型糖尿病小鼠NE，α1-AT蛋白表达的影响
- Author:
Wen SUN
1
;
Guang-yuan XU
2
;
Dan HOU
1
;
Li-li WU
1
Author Information
1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2. Fu Xing Hospital, Capital Medical University, Beijing 100038, China
- Publication Type:Research Article
- Keywords:
type 2 diabetes;
Tangnaikang;
ob/ob mice;
neutrophil elastase;
α1-antitrypsin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):22-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the regulatory effect of Tangnaikang (TNK) on imbalance between neutrophil elastase (NE) and α1-antitrypsin (α1-AT) in ob/ob mice with type 2 diabetes mellitus (T2DM). Method: Thirty-two male SPF ob/ob mice were randomly divided into model group (DM, normal saline) and high-dose TNK group (TNKH, TNK solution 16.04 g·kg-1), middle-dose TNK group (TNKM, TNK solution 8.02 g·kg-1) and low-dose TNK group (TNKL, TNK solution 4.01 g·kg-1). Another 8 C57BL/6J mice were included in normal group (Con, saline). The experiment lasted for four weeks. The general state, body weight (BW) and fasting blood glucose (FBG) of the mice were recorded weekly, the oral glucose tolerance (OGTT) test was performed on the 25th day, the insulin tolerance (ITT) test was performed on the 27th day, and the area under the curve (AUC) was calculated. After the end of the experiment, serum was used to detect the level of fasting insulin (Fins), insulin resistance index (HOMA-IR), total triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), NE and α1-AT. Adipose tissue was used to detect the expressions of NE, α1-AT, phosphor-insulin receptor substrate 1 antibody (p-IRS1) and glucose transporter 4 (GLUT4) proteins. Result: Compared with the Con group, the BW of the ob/ob mice of the model group increased significantly, the glucose and lipid metabolism indexes showed diabetes, the serum and adipose tissue NE increased significantly (P<0.01), and the α1-AT decreased significantly (P<0.01). Compared with the DM group, the BW, TG, TC, and LDL indexes in the TNKH group were significantly decreased (P<0.01), the HDL was significantly increased (P<0.01), and the FBG, Fins, HOMA-IR, AUCogtt and AUCITT were significantly decreased (P<0.01), serum and adipose tissue NE decreased significantly (P<0.01), α1-AT increased significantly (P<0.01), and adipose tissue p-IRS1 and GLUT4 increased significantly (P<0.05). Conclusion: TNK can reduce the BW of ob/ob mice, improve glycolipid metabolism, increase α1-AT level, decrease NE level, and regulate IRS1-GLUT4 signaling pathway, which may be one of its mechanisms in improving IR of adipose tissue mediated by neutrophil.
