Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β1 mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats
10.13422/j.cnki.syfjx.20192105
- VernacularTitle: 葛根散对酒精性ED大鼠阴茎平滑肌组织结构NOS活力及α-SMA,Cx43,TGF-β1 mRNA和蛋白表达的影响
- Author:
Qing-xue WANG
1
;
Qi-qi ZHANG
1
;
Chun-yan LIU
1
;
Shi-yan LUO
1
;
Yi-hui CHAI
1
Author Information
1. Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
- Publication Type:Research Article
- Keywords:
Gegensan;
alcohol;
erectile dysfunction;
nitric oxide synthase;
α-smooth actin;
gap junction protein gene 43;
transforming growth factor-β1
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the activity of nitric oxide synthase (NOS) and α-smooth actin (α-SMA) in rat penile smooth muscle tissue of rats with alcoholic erectile dysfunction (ED). The effects of protein gene 43 (connexin43, Cx43) and transforming growth factor β1 (TGF-β1) mRNA and protein expressions provide an experimental basis for the clinical application of Gegensan in the treatment of alcoholic ED. Method:SD rats were randomly divided into five groups:normal group, model group, and low,medium,high-dose Gegensan groups (5,10,20 g·kg-1). Except the normal group, the other groups were administered with drugs after alcohol intervention for 30 min at 15 mL·kg-1·d-1. Colorimetric assay was used to detect NOS activity in the penile smooth muscle tissue of alcoholic ED rats. Quantitative real-time fluorescence polymerase chain reaction(Real-time PCR) and Western blot were used to detect α-SMA, Cx43, TGF-β1 mRNA and protein expressions in smooth muscle tissue of alcoholic ED rats. Result:Compared with the normal group, the expressions of NOS, α-SMA and Cx43 mRNA and protein in the penile smooth muscle of the model group decreased significantly (P<0.05), while the expressions of TGF-β1 mRNA and protein increased significantly (P<0.05). Compared with the model group, low/medium/high-dose Gegensan groups could significantly up-regulate the NOS activity and Cx43 mRNA and protein expressions in the penile tissue of alcoholic ED rats (P<0.05), while significantly down-regulate TGF-β1 mRNA expression, and α-SMA mRNA and protein expressions in the penis tissue of rats with alcoholic ED were significantly up-regulated (P<0.05). Conclusion:Gegensan has an obvious protective effect on the structure of penile smooth muscle of alcoholic ED rats. The specific mechanism may be related to the regulation of NOS activity and a-SMA, Cx43 and TGF-β1 mRNA and protein expressions.
