Effect of Longshengzhi Capsule on Behavior and Cerebral Protection in Stroke Rats
10.13422/j.cnki.syfjx.20192403
- VernacularTitle: 龙生蛭胶囊对脑卒中大鼠行为学的影响及脑组织的保护作用
- Author:
Xiao-li SUN
1
;
Yong WANG
1
;
Jun-jie LIU
1
;
Chun LI
1
;
Shuang-jie YANG
1
;
Zhi-yin WANG
1
;
Nan-nan TAN
1
;
Wei WANG
1
Author Information
1. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Longshengzhi capsule;
stroke;
cerebral protection;
nuclear transcription factor-κB(NF-κB);
cysteine aspartate-specific protease-3(Caspase-3)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):23-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Longshengzhi capsule (LSZC) on behavior, serum biochemical index, nuclear transcription factor-κB(NF-κB) and cysteine aspartate-specific protease-3(Caspase-3) in stroke rats. Method:Ischaemic stroke was induced by the permanent middle cerebral artery occlusion (pMCAO). The male sprague-dawley rats with successful pMCAO modeling were randomly divided into five groups:model (pMCAO) group, nimodipine (12 mg·kg-1) group and LSZC groups (2.4, 1.2, 0.6 g·kg-1). A sham group also set in the study. The intragastric administration lasted for 14 days. The rats were tested for neurological deficits, grip strength, rotation tolerance. An open field test was also performed to evaluate the behavior. The pathology of the brain was studied by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining. In addition, the levels of malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) in rat serum were tested in the experiment. Immunohistochemistry (ICH) and Western blot were used to analyze the expressions of Caspase-3 and NF-κB. Result:Compared the model group, LSZC significantly improved the grip strength, rotation tolerance and spontaneous activity. LSZC markedly reduced infarct volume, water content of brain, neurological scores, body weight decline in MCAO stroke rats (P<0.05, P<0.01). LSZC significantly decreased the levels of NO and MDA, but increased SOD activity (P<0.05, P<0.01). LSZC recovered neuronal morphologic damage in MCAO rats, and down-regulated the expressions of Caspase-3 and NF-κB. Conclusion:LSZC treatment can improve neurological deficit function and protect the brain of stroke rats. This effect may be realized by down-regulating the expressions of NF-κB and Caspase-3.
