Effect of Shaoyao Gancaotang and Its Disassembled Prescriptions on Expressions of TRPV3, TRPV4 in Myocardium and Hypothalamus of Rats with Ultra-high Temperature
10.13422/j.cnki.syfjx.20192301
- VernacularTitle: 芍药甘草汤及其拆方对超高温环境大鼠心肌、下丘脑TRPV3,TRPV4表达的影响
- Author:
Feng-yun YAO
1
;
Lin WANG
1
;
Chun-hua LIU
1
;
Fei-yun WANG
1
;
Chao LIU
1
;
Jie-lin JIANG
1
;
Bing-zhi WANG
1
Author Information
1. College of Traditional Chinese Medicine(TCM), Jiangxi University of TCM, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Shaoyao Gancaotang;
ultra-high temperature;
transient receptor potential vanilloid 3;
transient receptor potential vanilloid 4;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):9-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Shaoyao Gancaotang and its disassembled prescriptions on the expressions of transient receptor potential vanilloid 3 (TRPV3) and transient receptor potential vanilloid 4 (TRPV4) in myocardium and hypothalamus of rats in the ultra-high temperature environment, in order to explore the mechanism of Shaoyao Gancaotang and its disassembled prescriptions against the high temperature. Method:Seventy five male SPF-grade SD rats were randomly divided into 5 groups according to their body weight, namely normal control group, high temperature normal control group, Shaoyao Gancaotang group (2.16 g·kg-1), single Paeoniae Radix Alba(PRA) group (1.08 g·kg-1) and single Glycyrrhizae Radix et Rhizoma(GRR) group (1.08 g·kg-1). Except normal control group, the remaining groups were fed in artificial climate boxes for 13 days. The normal control group and the high temperature normal control group were given the same volume of pure water. Western blot and quantitative real-time fluorescence polymerase chain reaction(Real-time PCR) were used to determine expressions of TRPV3, TRPV4 protein and mRNA in myocardium and hypothalamus of each group. Result:Compared with the normothermic control group, the expressions of TRPV3 protein, TRPV4 mRNA, TRPV3 protein and TRPV3 mRNA in the myocardium of the hyperthermic control group were significantly higher than those of the normal control group (P<0.05), and the expression of TRPV3 protein in the hypothalamus was significantly higher than that of the normal control group (P<0.05). Compared with the normal control group, the expressions of TRPV3, TRPV4 proteins in myocardium of rats in PRA group were significantly lower than those in the control group, and the expression of TRPV4 protein in myocardium of rats in Shaoyao Gancaotang group and GRR group was significantly lower than that in control group. The expression of TRPV4 mRNA in myocardium of rats in PRA group was significantly increased (P<0.05). Conclusion:TRPV3, TRPV4 channels in rat myocardium and hypothalamus were activated in the high temperature environment. The anti-hyperthermic mechanism of Shaoyao Gancaotang and its disassembled prescriptions was related to the regulation of TRPV3, TRPV4 channel protein and mRNA expressions in rat myocardium and hypothalamus.
